Mice lacking TYRO3, AXL and MER (TAM) receptor tyrosine kinases (RTKs) are male sterile. The mechanism of TAM RTKs in regulating male fertility remains unknown. In this study, we analyzed in more detail the testicular phenotype of TAM triple mutant (TAM−/−) mice with an effort to understand the mechanism. We demonstrate that the three TAM RTKs cooperatively regulate male fertility, and MER appears to be more important than AXL and TYRO3. TAM−/− testes showed a progressive loss of germ cells from elongated spermatids to spermatogonia. Young adult TAM−/− mice exhibited oligo-astheno-teratozoospermia and various morphological malformations of sperm cells. As the mice aged, the germ cells were eventually depleted from the seminiferous tubules. Furthermore, we found that TAM−/− Sertoli cells have an impaired phagocytic activity and a large number of differentially expressed genes compared to wild-type controls. By contrast, the function of Leydig cells was not apparently affected by the mutation of TAM RTKs. Therefore, we conclude that the suboptimal function of Sertoli cells leads to the impaired spermatogenesis in TAM−/− mice. The results provide novel insight into the mechanism of TAM RTKs in regulating male fertility.
Many male infertility cases are diagnosed as idiopathic. The molecular mechanisms underlying these defects largely remain to be elucidated. By gene targeting technology, male infertility has been demonstrated as a phenotype of the deficiency in various single genes (Matzuk & Lamb 2002). These genes encode different classes of proteins including cytoskeletal proteins, transcription factors, signal transduction molecules, metabolic enzymes, and membrane proteins. Here, we investigate the role of TAM receptor tyrosine kinases (RTKs) in regulating male fertility.
RTKs, a superfamily of cell surface receptors with a common highly conserved intracellular tyrosine kinase domain (Robinson et al. 2000), are responsible for transmembrane signal transduction after binding of the extracellular domains to their ligands. They are critical for regulation of survival, proliferation and differentiation. Twenty subfamilies of RTKs have been categorized so far based on their amino acid sequence identities and extracellular structural similarities. The members of a subfamily bind common or similar ligands (Manning et al. 2002). The TAM RTK subfamily was identified most recently and contains three members – TYRO3, AXL, and MER (Hafizi & Dahlback 2006b). Two highly homologous vitamin K-dependent proteins, product of growth arrest-specific gene 6 (Gas6) and protein S (a negative regulator of blood coagulation) are the common ligands of TAM RTKs (Hafizi & Dahlback 2006a). Studies using gene knockout models have provided direct insights into the physiological functions of TAM RTKs (Lemke & Rothlin 2008). Mice lacking any single receptor or any combination of two members were viable and capable of producing apparently healthy offspring. However, the TAM triple mutant mice displayed multiple major defects, including broad spectrum autoimmune diseases (Lu & Lemke 2001, Lemke & Lu 2003), impaired natural killer cell differentiation (Caraux et al. 2006), hematopoietic abnormalities (Wang et al. 2007, Tang et al. 2009) and male infertility (Lu et al. 1999), suggesting that the three members of TAM RTK subfamily function redundantly or cooperatively.
Attention deserves to be paid to the role of TAM RTKs in regulating male fertility. Mammalian spermatogenesis is characterized by continuous germ cell maturation through various stages including mitotic proliferation of spermatogonia, meiotic division of spermatocytes, and morphogenesis of spermatids (spermiogenesis). During this process, more than 75% of developing spermatogenic cells undergo apoptosis before they mature into spermatozoa in physiological conditions (Dunkel et al. 1997). In the last stage of spermatogenesis, the cytoplasmic portions of elongated spermatids are shed to form residual bodies before extrusion of differentiated sperm into the lumen of seminiferous tubules (Kerr & de Kretser 1974). The phagocytic clearance of the apoptotic germ cells and residual bodies by Sertoli cells is critical to keeping integrity of the seminiferous epithelium and healthy spermatogenesis (Maeda et al. 2002). The molecular mechanisms of Sertoli cell phagocytosis have not been extensively studied. In the present study, we analyzed the functions of TAM RTKs in regulating spermatogenesis using mice mutant for a series of TAM RTKs. We describe previously unrecognized testicular phenotypes in TAM−/− mice, which may provide novel insights into the mechanism underlying the impaired spermatogenesis due to the mutation of TAM RTKs.
Expression of TAM RTKs in testicular cells
To examine the distribution of TAM RTKs in testis, immunohistochemistry on testicular sections was carried out using antibodies against the three proteins. As shown in Fig. 1A and B, TYRO3 and AXL were localized to Sertoli cells; MER was detected both in interstial Leydig cells (LCs) and Sertoli cells (upper panel). In order to confirm the cell-specific expression of TAM RTKs, we performed immunocytochemistry on the isolated primary testicular cells. AXL and TYRO3 proteins were exclusively expressed by Sertoli cells, whereas MER was evidently expressed by both LCs and Sertoli cells. Notably, no signal for TAM RTKs was detected in spermatogenic cells. Consistently, the three TAM RTKs were detected in Sertoli cells by western blotting, MER was also observed in LCs, and none of them were found in germ cells (Fig. 1B). To verify the purity of the testicular cell types, the three cell fractions were characterized by cell type specific markers (Wilm's tumor nuclear protein 1 (WT1) for Sertoli cells, VASA for germ cells, 3β-hydroxysteroid dehydrogenase 1 (HSD3B1) for LCs). As shown in Fig. 1C, each cell fraction specifically expressed the cell type marker, indicating that the cell fractions were not contaminated by other cell types. Notably, TAM RTKs were absent in Sertoli cells isolated from TAM−/− mice (data not shown), confirming that TAM RTKs alleles are null in the triple mutant mice.
Fertility of TAM RTKs mutant mice
To dissect the role of each TAM RTK on male fertility, TAM RTK single, double and triple mutant mice were examined on their fertility, testis development and sperm count. The results are listed in Table 1. No significant difference in the body weight of mice carrying different genotypes was observed. However, significant decreases in the testis weight, sperm production and fertility were found in mice with some genotypes. As expected, the most severe phenotype was observed in the triple knockouts. TAM−/− mice never sired offspring. The weight of testis in TAM−/− mice was one third that in WT controls at 15 weeks of age. Very few sperm were found in TAM−/− epididymis. Although all other genotype mice were fertile, the mice doubly mutant for Axl/Mer (AM−/−) and Tyro3/Mer (TM−/−) exhibited significant decreases in testis weight, sperm count, and fertility compared with WT mice. In contrast, all single mutant mice and Tyro3/Axl double mutants (TA−/−) exhibited normal fertility. These results indicate that the three TAM RTKs cooperatively regulate spermatogenesis. That all mice with compromised fertility carry the Mer null allele suggests that MER is more important than AXL and TYRO3 in regulating male fertility.
Male fertility-related phenotypes of mice with different genotypes.
|Genotypes of male mice||Body weight (g)a||Testis weight (mg)a||Number of sperm (×107/epididymis)a||Fertility (offsprings/cage)b|
Data are presented as means±s.e.m. of 10-week-old mice (n=10).
Fertility is presented as means±s.e.m. of 3 cages during mating of TAM−/− male and WT female for 6 months. A−/−, T−/−, and M−/− represent Tyro3, Axl, and Mer single mutants respectively, AM−/− represents Axl/Mer double mutants, and so forth. TAM−/− and WT represent Tyro3/Axl/Mer triple mutant and wild-type mice.
Progressive loss of germ cells during post-natal development of TAM−/− testis
Since TAM RTKs triple knockouts were male sterile, we then further examined the spermatogenesis of TAM−/− mice (Fig. 2). We found a progressive loss of germ cells as TAM−/− mice aged. At week 2 after birth, histological appearance of the testes from both TAM−/− and WT mice was similar, the seminiferous tubules contain Sertoli cells, spermatogonia and primary spermatocytes in number comparable. On week 3, the most tubules of TAM−/− and WT testes showed the presence of round spermatids. However, a delayed progression of germ cell differentiation was occasionally observed in some tubules of TAM−/− testis, where the most advanced germ cells were late-stage spermatocytes. On week 8, the elongated spermatids were much less in TAM−/− tubules than WT controls. Frequently, multiple steps of spermatids appeared on a same tubular section, suggesting an unsynchronized spermatogenesis. These observations indicate that the maturation of germ cells in the first wave of spermatogenesis in TAM−/− mice is delayed when spermatocytes proceed to spermatids. The impaired spermatogenesis became more severe as mice aged. At week 15, multinucleate giant cells containing multiple nuclei within a single cytoplasm were frequently observed in TAM−/− seminiferous tubules, indicating a defect on the breaking up cytoplasmic bridges between sister spermatids. Although all stages of germ cells were present in most TAM−/− tubules at 15 weeks of age, many fewer spermatids and spermatocytes were observed compared to WT controls. Meanwhile, the vacuoles were frequently seen in the seminiferous epithelium. Further loss of the early stage of germ cells occurred thereafter. Up to 30 weeks of age, almost all the seminiferous tubules were completely devoid of germ cells in TAM−/− testes. Consistent with these observations, the epididymides of TAM−/− mice were filled with sparse spermatozoa and abundant degenerated round cells at 8 and 15 weeks of age (Fig. 2). At 30 weeks of age, the TAM−/− epididymides contained only sparse degenerated round cells. In controls, the epididymides of adult WT mice were filled with plenty of spermatozoa, and very few round cells were observed. Taken together, the observations demonstrate that the germ cells were progressively lost from spermatids to spermatocytes, and finally spermatogonia as TAM−/− mice aged.
Apoptosis and proliferation of germ cells
To clarify whether the germ cell loss is due to cell apoptosis or defective proliferation, we analyzed cell apoptosis and proliferation based on TUNEL assay and immunostaining for proliferating cell nuclear antigen (PCNA). We found massive apoptotic spermatids in the testis of 10-week-old TAM−/− mice (Fig. 3A). As mice aged, more apoptotic early stage of germ cells appeared. Apoptotic germ cells were rarely observed in adult WT mice. On the other hand, we did not find evident impaired proliferation of spermatogonia in adult TAM−/− mice (Fig. 3B). The results suggest that the loss of germ cells in TAM−/− testis mainly results from the increased cell apoptosis, not absence of spermatogonia proliferation. Consistent with the loss of germ cells, the seminiferous tubule diameter was significantly decreased in TAM−/− mice at 15 weeks of age and older (Fig. 3C). The decreased tubule diameter should reflect a total germ cell complement.
Oligo-astheno-teratozoospermia in young adult TAM−/− mice
Although TAM−/− males never sired offspring, spermatozoa appeared in the testes and epididymides of young adult mice. However, the number of epididymal spermatozoa in 10-week-old TAM−/− mice is 12% of that in WT controls (Fig. 4A). Moreover, only 15% of TAM−/− spermatozoa were motile (Fig. 4B), and the motile spermatozoa beat less vigorously and generated less forward momentum. Notably, most of the spermatozoa exhibited morphological malformation. The more prevalent shapes were depicted in Fig. 4C: with a head being curled back onto the midpiece (a), with a coiled of tail (b), having two heads (c and d), and multiple spermatozoa bundled by unidentified material (e and f); <10% of spermatozoa showed normal morphological features (g). Male infertility of TAM−/− mice could be attributable to oligo-astheno-teratozoospermia. The malformation of spermatozoa was further revealed by electron microscopic examination (Fig. 4D). Compared to controls (a and b), the most epididymal spermatozoa of TAM−/− mice showed a deformity of head shape characterized by their irregular acrosomes, which were expanded or partially detached from the nucleus membrane (c and d). Notably, most mitochondria failed to arrange helicoidally around the axoneme in the middle piece of spermatozoa (e and f). The mitochondria were completely absent in some sperm (g). The deformation of mitochondrial sheaths might be responsible for the defective motility of TAM−/− spermatozoa.
Impaired phagocytic ability of TAM−/− Sertoli cells
Given that all TAM RTKs were expressed in Sertoli cells, not germ cells, it was reasonable to speculate that they primarily act on Sertoli cells and thus regulate spermatogenesis. Therefore, the characteristics of Sertoli cells were examined in TAM−/− mice. Based on immunostaining for WT1 (a nuclear marker of Sertoli cells) (Fig. 5A), the number of Sertoli cells was comparable (17.1±0.22 vs 16.4±0.18/tubular section) in TAM−/− and WT seminiferous tubules at 8 weeks of age. At 30 weeks of age, although most of the seminiferous tubules in TAM−/− testis contain only Sertoli cells, the number of Sertoli cells was still consistent within TAM−/− and WT tubules (17.6±0.23 vs 17.9±0.29/tubular section). The results indicate that the mutation of TAM RTKs does not affect survival of Sertoli cells. In order to assess the maturation state of TAM−/− Sertoli cells, we examined the expression of major differentiation markers in isolated primary Sertoli cells by quantitative RT-PCR. The androgen receptor, androgen binding protein and anti-Müllerian hormone were comparably expressed at the mRNA level in WT and TAM−/− Sertoli cells (Fig. 5B), suggesting that Sertoli cells can normally mature in the mutants.
One of the Sertoli cell's functions is the phagocytic clearance of apoptotic germ cells and residual bodies, which is necessary for normal spermatogenesis. We recently demonstrated in vitro that GAS6 and TAM RTKs regulate the phagocytosis of apoptotic germ cells by Sertoli cells (Xiong et al. 2008). To examine whether the mutation of TAM RTKs in Sertoli cells impairs their phagocytic function in vivo, we analyzed dynamics of lipid droplet formation in the seminiferous epithelium during testis development postnatal. Lipid droplets can be formed by Sertoli cells after engulfment of apoptotic germ cells and residual bodies, which can be a criterion to evaluate phagocytic ability of Sertoli cells (Wang et al. 2006). By Oil Red O (ORO) staining on testis sections, we found an increased lipid droplet number in basal compartment of the seminiferous epithelium during development of WT testes (Fig. 5C). However, the number of lipid droplets in TAM−/− seminiferous tubules was significantly lower compared with that in WT controls at 3 weeks of age. In 8-week-old WT mice, the lipid droplets showed stage-dependant variation with a peak number at the stages IX–I (Fig. 5C), where phagocytosis of residual bodies take places. Notably, significantly fewer lipid droplets were observed at the same stage of TAM−/− tubules, although massive degenerated spermatogenic cells appeared in the tubules. The decreased lipid droplets could reflect an impaired phagocytotic ability of TAM−/− Sertoli cells. This result is agreement with that obtained in our previous in vitro study (Xiong et al. 2008).
Characteristics of LCs in TAM−/− testis
Since MER was also evidently expressed in interstitial LCs, we asked whether the mutation of TAM RTKs affects the function of LC. Therefore, the characteristics of LCs were compared in WT and TAM−/− testes. LCs were isolated from TAM−/− and WT testes, and stained for HSD3B1 (Fig. 6A). By counting HSD3B1-positive cells at different ages of mice, we found a comparable LC number in TAM−/− and WT testes (Fig. 6B). LC number increased sharply from 3 to 5 weeks of age, and remained consistent thereafter. One important function of LCs is the production of testosterone, which plays a critical role in maintaining spermatogenesis. RIA showed similar serum testosterone levels (Fig. 6C) in 15-week-old TAM−/− and WT mice. Furthermore, based on the data of microarray analysis, we did not find differential expression of the genes that are specifically expressed in LC and play critical roles in regulating spermatogenesis in WT and TAM−/− testes (Supplementary Table 1, which can be viewed online at www.reproduction-online.org/supplemental/), such as LH receptor and three steroidogenic enzymes (HSD3B1, p450c17 and p450scc). The results suggest that the maturation and functions of LC are not apparently affected by the lack of TAM RTKs.
Gene expression profile of TAM−/− testes
To further understand the molecular consequences of TAM RTK mutation in testis, we analyzed the gene expression profile of TAM−/− and WT testes using a mouse microarray (GeneChip Mouse Genome 430/2.0, Affymetrix, Santa Clara, CA, USA) containing a total of 39 000 probe sets targeting all known mouse gene transcripts. The testes from 3-week-old mice were used for the microarray analysis, because there are similar testicular cell fractions in the testes of TAM−/− and WT mice at this age. The genes that are up- and downregulated by more than twofold in TAM−/− testes compared to WT controls were 287 and 149 respectively (Supplementary Table 1). Notably, TAM RTKs were listed as the mostly downregulated genes, indicating that the microarrary system works well. Onto-Express was used to classify the differentially expressed genes according to their biological functions (Fig. 7A).
Furthermore, we selected several differentially expressed genes based on their predominant expression in Sertoli cells as well as possible biological significance, and verified their expression in isolated primary Sertoli cells by real-time PCR. We confirmed the differential expression of three upregulated and three downregulated genes in TAM−/− Sertoli cells (Fig. 7B). Notably, the expression of N-cadherin was significantly reduced in TAM−/− Sertoli cells. N-cadherin is critical for spermatogenesis by mediating the formation of ectoplasmic specialization (EP) between Sertoli cells and spermatids. The down-regulation of N-cadherin in TAM−/− Sertoli cells might perturb EP formation and thus impaired spermatid maturation. The expression of Cd36, a scavenger receptor in Sertoli cells to mediate the phagocytosis of apoptotic spermatogenic cells, was also significantly downregulated in TAM−/− Sertoli cells, which might contribute to the impaired phagocytic ability of TAM−/− Sertoli cells. Among the upregulated genes, tumor necrosis factor-α (Tnf) was upregulated by 12-fold in TAM−/− Sertoli cells. The roles of TNF in regulating survival and death of germ cells were controversial. The marked up-regulation of Tnf in TAM−/− Sertoli cells could involve in the impaired spermatogenesis. The expression of lactate dehydrogenase C (Ldhc) was increased by 18-fold in TAM−/− Sertoli cells, suggesting a changed metabolism due to TAM RTKs mutation. Although an abrogation of Mer expression in TAM−/− Sertoli cells was expectable, we surprisingly confirmed a significant up-regulation of Gas6 in the mutant Sertoli cells. The gene expression profile of TAM−/− testis could provide clues to understand the molecular mechanisms of TAM RTKs in regulating spermatogenesis.
A previous study demonstrated that TAM RTK triple mutant male mice are sterile (Lu et al. 1999). Here we analyzed in detail the functions of TAM RTKs in regulating male fertility and possible mechanisms underlying the defects due to TAM RTK mutation. The results expand the previous report and provide novel insight into the roles of TAM RTKs in regulating spermatogenesis.
We found that the three TAM RTKs regulate male fertility in a cooperative manner. Mer knockout is necessary for the impaired fertility in the double mutant mice, suggesting that MER may be more important than TYRO3 and AXL in regulating male fertility. Impaired germ cell maturation in TAM−/− mice was observed at the first wave of spermatogenesis, and became more severe as the mice aged. In young adult TAM−/− mice, this defect is characterized by an impaired spermiogenesis. During spermiogenesis, the round spermatids synchronously elongate, condense their nuclei, acquire flagellar and acrosomal structures, and shed a great portion of their cytoplasm (as residual bodies) to form spermatozoa (Cheng & Mruk 2002). Defects of these events leads to a lack of mature spermatozoa, which is a major cause of male infertility in humans (Ezeh 2000). The round spermatids in the young adult TAM−/− mice mostly failed to elongate. The presence of multinucleate giant cells suggests a failure in the shedding of residual bodies and the breaking up of intercellular bridges between sister spermatids. Oligo-astheno-teratozoospermia has been observed in the mice mutant for other genes such as Rxrb (Kastner et al. 1996), Cont-7 (Nakamura et al. 2004) and Tslc1 (van der Weyden et al. 2006, Yamada et al. 2006). The young adult TAM−/− mice exhibited severe oligo-astheno-teratozoospermia. Various morphological malformations of sperm were found in TAM−/− mice. Malformed sperm were observed in some infertile men with teratozoospermia (Baccetti et al. 1979, Lewis-Jones et al. 2003). The observations in this study could provide novel insight into the etiology of oligo-astheno-teratozoospermia. Electron microscopy showed malformed heads and mitochondria sheaths in TAM−/− spermatozoa, which could contribute to the asthenospermia. The sperm mitochondria deformation was reported in Cont7 null mice, and the head abnormalities were also observed in Tslc1 and nectin-2 null mice (Mueller et al. 2003). Our present study provides a novel example of oligo-astheno-teratozoospermia, which results from the mutation of TAM RTKs.
Impaired spermatogenesis should not be germ cell autonomous in TAM−/− mice because no one TAM RTK was expressed in the germ cells. In contrast, all three TAM RTKs were co-expressed in Sertoli cells, suggesting that they primarily affect Sertoli cells and thus regulate spermatogenesis. Sertoli cells are critical for normal spermatogenesis. Germ cell survival during the first wave of spermatogenesis is lower than in subsequent waves probably because Sertoli cell function is not optimal unless it is exposed to a full complement of germ cells. The germ cell degeneration and teratozoospermia due to the suboptimal Sertoli cell function have been documented in a number of mouse mutants (Kastner et al. 1996, Russell et al. 2001, Firestein et al. 2002, Nakamura et al. 2004, Ogawa et al. 2004). A recent study demonstrated that dimeric transferrin in Sertoli cells modulates the phagocytosis of residual bodies (Yefimova et al. 2008). We demonstrated in this study that the survival and differentiation of Sertoli cells are not affected by the mutation of TAM RTKs, but their phagocytic function is impaired. Normal phagocytic function of Sertoli cells is necessary for healthy spermatogenic cells to proceed through spermatogenesis (Maeda et al. 2002). One explanation for how the timely removal of apoptotic cells and residual bodies contributes to spermatogenesis is the prevention of leaking out noxious contents and poisoning healthy cells (Nakanishi & Shiratsuchi 2004). We recently demonstrated that the apoptotic spermatogenic cells and residual bodies can be used to produce energy by Sertoli cells after the phagocytosis of them (Xiong et al. 2009). The net output of energy by Sertoli cells is important not only to spermatogenesis, but also to germ cell movement (Mruk & Cheng 2004). Impaired Sertoli cell phagocytosis may result in a decrease in the energy production by Sertoli cells. On the other hand, although MER was also evidently expressed in LCs, we did not observe any apparent defect of LC functions that are critical for spermatogenesis due to lacking TAM RTKs. Therefore, the defect of Sertoli cell phagocytosis could be a major cause of the impaired spermatogenesis in TAM−/− mice.
We finally compared the gene expression profiles of TAM−/− and WT testes. A large number of differentially expressed genes were found in TAM−/− testes compared to WT controls. The number of upregulated genes was almost two times that of downregulated genes in TAM−/− testes (287 vs 149), which is agreement with the knowledge that TAM RTKs negatively regulate various transcription factors including nuclear factor κB (NFKB) activation (Lemke & Rothlin 2008). N-cadherin was one of the most profound downregulated genes in TAM−/− Sertoli cells. It has been known that N-cadherin mediates Sertoli cell–spermatid adhesion (Newton et al. 1993). The down-regulation of N-cadherin might perturb the adhesion between Sertoli cells and spermatids, thus result in a primary damage of spermiogenesis in TAM−/− mice. CD36 is a regulator of the phagocytosis of apoptotic germ cells by Sertoli cells (Gillot et al. 2005). A significant down-regulation of Cd36 might contribute to the defective phagocytic ability of TAM−/− Sertoli cells. We did not find the differential expression of other phagocytosis-related genes in TAM−/− testis based on the microarray data. Ldhc, a key enzyme in glycolysis, was upregulated by 18-fold in TAM−/− Sertoli cells. We recently reported that lipids formed by Sertoli cells after engulfing apoptotic germ cells can be an energy source for Sertoli cells (Xiong et al. 2009). The impaired phagocytic ability of TAM−/− Sertoli cells might inhibit this metabolic pathway. The up-regulation of LDHC could be a compensatory response to the decreased lipid metabolism. Tnf was another potentially interesting upregulated gene in TAM−/− Sertoli cells. It has been reported that TNF produced by Sertoli cells in rat testis with experimental autoimmune orchitis can triggers germ cell apoptosis (Suescun et al. 2003). The marked up-regulation of Tnf in TAM−/− Sertoli cells might involve in the impaired spermatogenesis. The expression of TNF by Sertoli cells and its biological function in spermatogenesis in pathological condition has not been extensively studied, and should be worthwhile to investigate. Although TAM RTKs were completely inactivated in TAM−/− Sertoli cells, the expression of their common ligand (Gas6) was significantly upregulated. This observation raised another interesting issue: the GAS6/TAM system may regulate its own gene expression to balance their biological function; we are investigating this possibility.
In conclusion, we analyzed in detail the phenotypes of male fertility and spermatogenesis duo to lacking TAM RTKs in mice, and demonstrated some mechanisms probably underlying the observed histological defects. The data provide novel insight into the roles of TAM RTKs in regulating spermatogenesis and male fertility.
Materials and Methods
Mice singly mutant for TAM RTKs were kindly provided by Dr Lemke (Salk Institute for Biological Studies, La Jolla), and were progeny of the original colony with a genetic background of 50% 129/sv×50% C57BL/6. Double and triple mutant mice were produced by cross mating of the single mutants. The wild-type control mice were the littermates of the mutant mice. The animals had free access to food and water, and were handled in compliance with the guideline for the care and use of laboratory animals established by the Chinese Council on Animal Care.
To assess male fertility, 8-week-old mutant males were mated with wild-type females at a ratio of 1:1 in cages. Three cages were set for each genotype. Total litter number was recorded for 6 months, and mean number of the three cages each genotype was presented in Results.
Testes and epididymides were removed from mice and embedded in paraffin. After fixation in Bouin's solution, a serial of sections of 5 μm in thickness were cut for appropriate staining after deparaffinization and dehydrations. The tubule diameter was measured under light microscope. Three mice each group were used for histological analysis. At least 150 seminiferous tubule sections from three animals were examined.
Isolation of Sertoli, Leydig and spermatogenic cells
The procedure for the isolation of Sertoli cells, LCs and spermatogenic cells was based on a previous description (Wang et al. 2006). Briefly, decapsulated testes were incubated with 0.5 mg/ml of collagenase (Sigma) at room temperature for 15 min with gentle oscillation, and then were filtered through 100 μm copper meshes to collect interstitial cells and seminiferous tubules.
The interstitial cells were cultured in F12/DMEM medium (Gibco) containing 10% FCS in an incubator containing 5% CO2 at 32 °C. After 24 h, the interstitial cells were treated by 0.125% trypsin for 5 min to collect LCs. The LCs were counted and reseeded to examine the purity by staining for HSD3B1.
Seminiferous tubules were resuspended in the collagenase at room temperature for an additional 20 min to remove myoid cells. Then, the tubules were cut into small pieces and incubated with 0.5 mg/ml of hyaluronidase (Sigma) for 15 min with gentle oscillation and pipetting. The cell suspensions were cultured in F12/DMEM supplemented with 10% FCS at 32 °C for 24 h. Non-adherent spermatogenic cells were collected, and those adhered to Sertoli cells were removed by a treatment with hypotonic solution (20 mM Tris, pH 7.4) for 1 min. Twenty-four hours later, Sertoli cells were recovered by treatment with 0.125% trypsin for 5 min, and reseeded to evaluate the purity by staining for WT1 (a marker of Sertoli cells).
For immunohistochemistry, the testicular sections were incubated in PBS containing 3% H2O2 for 15 min to block endogenous peroxidase activity, and then soaked in a citrate buffer and microwaved at 100 °C for 10 min for antigen retrieval. After blocking with 5% normal bovine serum, the sections were incubated overnight at 4 °C with the primary antibodies purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), polyclonal antibodies against TYRO3 (SC1095), AXL (SC1096), MER (SC6873), WT1 (SC192), and PCNA (SC7907). After rinsing with PBS, the sections were incubated with the biotinylated secondary antibody, goat anti-rabbit IgG (Zhongshan, Beijing, China), at room temperature for 30 min. The streptavidin-peroxidase activity was visualized with diaminobenzidine method. Negative controls were incubated with preimmune serum instead of primary antibodies. The sections were counterstained with hematoxylin and mounted with Canada balsam (Sigma) for observation.
For immunocytochemistry, Sertoli cells and LCs were cultured in Lab-Tek Chamber Slide (Nunc, Naperville, IL, USA; four chambers per slide), germ cells were spun onto slides. After washing twice with PBS, cells were fixed with cold methanol at −20 °C for 1 min. The endogenous peroxidase activity was blocked by a treatment with 3% H2O2 in methanol for 15 min. Then, cells were permeabilized by a 15 min treatment with 0.3% Triton X-100 in PBS. After preincubation with 5% normal bovine serum in PBS at room temperature for 1 h, the slides were incubated with the polyclonal antibodies against TYRO3, AXL, and MER at room temperature for 90 min. The succeeding procedures were identical to the corresponding ones in immunohistochemistry staining.
Total cell lysates were prepared with cell lysis buffer (BioDev-Tech, Beijing, China). Protein concentration was measured using the micro-bicinchonic acid method (Pierce Biotechnology, Rockford, IL, USA). Equal amount of proteins were subject to SDS-PAGE, and electrotransferred onto PVDF membrane (Millipore, Bedford, MA, USA). After blocking with 5% nonfat dry milk in Tris-buffered saline (TBS) for 1 h, the membranes were incubated with primary antibodies (Santa Cruz) at appropriate dilutions at 4 °C overnight: polyclonal antibodies against TYRO3, AXL, MER, WT1, VASA (SC97185), and HSD3B1 (SC30821). After washing twice with TBS, the membranes were incubated with appropriated peroxidase-conjugated affinipured secondary antibodies (Zhongshan) at room temperature for 1 h. After washing twice with TBS, antigen–antibody complex was visualized using an enhanced chemiluminescence detection kit (Zhongshan).
Apoptotic cells were detected using TUNEL staining kit (Zhongshan) based on the manufacture's instructions. Briefly, after blocking endogenous peroxidase as description above, the testicular sections were incubated with terminal deoxynucleotidyl transferase labeling for overnight at 4 °C. After washing with PBS, the sections were incubated with anti-FITC-HRP conjugate at 37 °C for 2 h, and then visualized with diaminobenzidine. The sections were counterstained with hematoxylin.
Analysis of spermatozoa
Epididymides were removed from 10-week-old mice, and cut to 1-mm-long pieces in DMEM medium with 10% FCS. Then the small pieces were homogenized to dissociate somatic cells, then incubated at 32 °C for 10 min to allow dispersion of sperm. The sperm number was counted with a hemocytometer. The sperm motility was examined as previously described (Baker et al. 1996).
Epididymal sperm were pelleted by centrifugation for 5 min at 1000 g, and fixed in 2.5% glutaraldehyde (Sigma) in PBS at 4 °C for 2 h. After washing twice with PBS, the pellets were postfixed in 1% osmium tetroxide (Fisher Scientific Co., Fair Lawn, NJ, USA) for an additional 2 h at 4 °C. After dehydrating with graded acetone, the pellets were embedded using an araldite embedding kit (Polysciences Inc., Warrington, PA, USA). Ultrathin sections (70 nm in thickness) were cut and placed on formvar-coated copper grids, stained with uranyl acetate and lead citrate, and viewed with a Jem 1200 Ex transmission electron microscope (JEOL, Tokyo, Japan).
To examine lipid droplets in testis, frozen sections (8 μm in thickness) were cut and mounted on poly-l-lysine coated slides. The sections were fixed with 10% neutral-buffered formalin for 15 min. After a wash with 70% ethanol for 1 min, the sections were incubated with ORO solution (ORO saturated solution in isopropanol:water=3:2, Sigma) for 15 min. The sections were washed with 70% alcohol for 10 s to remove background staining, then were counterstained with Harris' hematoxylin and mounted in glycerol: PBS at 9:1 (v/v). Lipid droplets were visualized and counted under a microscope (IX71, Olympus).
Staining for HSD3B1
Staining for HSD3B1 was carried out based on a method described by Klinefelter et al. (1987). Briefly, LCs were fixed with 10% formalin in PBS and dried for 10 min at room temperature. Then the cells were stained with a mix of solution A (1 mg NBT dissolved in 0.6 ml of 1 mg/ml etiocholan-3β-ol-17-one in DMSO) and solution B (10 mg β-NAD+ in 9.5 ml PBS) for 90 min. After rinsing in distilled water, the slides were mounted with glycerol:PBS=1:1 (v/v).
Measurement of testosterone
Blood samples were collected by bleeding from tail of 15-week-old mice, and sera were collected by centrifugation at 1500 g for 10 min. The testosterone level in serum was measured by RIA as previously described (Suescun et al. 1985). Five animals from each group were examined and data were presented as mean±s.e.m.
Microarray analysis was performed according to the protocol of Affymetrix's instructions. Briefly, total RNA was extracted from the testes of 3-week-old WT and TAM−/− mice using Trizol reagent (Invitrogen), and reversely transcribed into the first strand cDNA with superscript II reverse transcriptase using a T7-Oligo(dT) promoter primer. Following RNase H-mediated second-strand cDNA synthesis, the double-stranded cDNA was purified and served as a template in the subsequent in vitro transcription reaction, which was carried out in the presence of T7 RNA polymerase and a biotinylated nucleotide analog for cRNA amplification and biotin labeling. The biotinylated cRNA targets were cleaned up with an RNeasy mini column (Qiagen), fragmented to 50–200 nucleotides, and hybridized to GeneChip Mouse Genome 430/2.0 (Affymetrix). After staining with Fluidics Station 450, the microarrays were scanned with a GeneChip Scanner 3000, and the signals were analyzed with GeneChip Operating Software (GCOS 1.4). The data from duplicates on the arrays were fused. Changes of more than twofold in intensities were considered differentially expressed genes, which were listed in Supplementary Table 1 and classified using Onto-Express according to their gene ontology categories.
Sertoli cells were isolated from the testes of 3-week-old mice, total RNAs were extracted from the cells using Trizol reagent according to the manufacturer's instructions. The RNA (1 μg) was reversely transcribed into cDNA in 20 μl of RT reaction mixture containing 2.5 μM random hexamers, 2 mM dNTPs and 200 U M-MLV reverse transcriptase (Promega). Real-time PCR was performed with Power SYBR Green PCR master mix kit (Applied Biosystems, Foster City, CA, USA) using an ABI PRISM 7300 real-time cycle (Applied Biosystems). The mRNA levels of target genes were normalized to 28s rRNA. The primers for real-time PCR were listed in Table 2.
Primers used for real-time PCRs.
|Primer pairs (5′–3′)|
Ldhc, lactate dehydrogenase C; Tnf, tumor necrosis factor-α; Abp, androgen binding protein; Ar, androgen receptor; Amh, anti-Müllerian hormone; 28s rRNA, 28s ribosome RNA.
For all comparisons, statistical analysis was carried out by Student's t-test to generate P values. Statistical significance was defined as P<0.05. The calculations were performed with SPSS version 11.0 statistic software.
Declaration of interest
The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
This work was supported by Special Funds for Major State Basic Research Project of China (grant no. 2007CB947504, 2006CB504001).
We sincerely thank Dr Greg Lemke for providing TAM RTK mutant mice.
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