Inhibition of TMEM16A impedes embryo implantation and decidualization in mice

in Reproduction
Authors:
Qianrong Qi Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China

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Yifan Yang Medical College of Wuhan University, Wuhan, People’s Republic of China

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Kailin Wu Medical College of Wuhan University, Wuhan, People’s Republic of China

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Qingzhen Xie Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China

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Correspondence should be addressed to Q Xie; Email: drqingzhenxie@126.com
DOI:
https://doi.org/10.1530/REP-18-0197
Volume/Issue:
Volume 156: Issue 6
Page Range:
569–577
Article Type:
Research Article
Online Publication Date:
Dec 2018
Copyright:
© 2018 Society for Reproduction and Fertility 2018
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Recent studies revealed that TMEM16A is involved in several reproductive processes, including ovarian estrogen secretion and ovulation, sperm motility and acrosome reaction, fertilization and myometrium contraction. However, little is known about the expression and function of TMEM16A in embryo implantation and decidualization. In this study, we focused on the expression and regulation of TMEM16A in mouse uterus during early pregnancy. We found that TMEM16A is upregulated in uterine endometrium in response to embryo implantation and decidualization. Progesterone treatment could induce TMEM16A expression in endometrial stromal cells through progesterone receptor/c-Myc pathway, which is blocked by progesterone receptor antagonist or the inhibitor of c-Myc signaling pathway. Inhibition of TMEM16A by small molecule inhibitor (T16Ainh-A01) resulted in impaired embryo implantation and decidualization in mice. Treatment with either specific siRNA of Tmem16a or T16Ainh-A01 inhibited the decidualization and proliferation of mouse endometrial stromal cells. In conclusion, our results revealed that TMEM16A is involved in embryo implantation and decidualization in mice, compromised function of TMEM16A may lead to impaired embryo implantation and decidualization.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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