Effects of intravenous infusion of E. coli lipopolysaccharide in early pregnant cows

in Reproduction
Correspondence should be addressed to K Herzog; Email: kathrin.herzog@laves.niedersachsen.de

*(K Herzog and L Debertolis contributed equally to this work)

(K Herzog is now at Lower Saxony State Office for Consumer Protection and Food Safety, Oldenburg, Germany)

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The objective was to characterize effects of Escherichia coli LPS (given i.v.) on corpus luteum (CL) and embryonic viability in early pregnant cattle. Eight non-lactating German Holstein cows were given 0.5 µg/kg LPS on 35 ± 3 day (mean ± s.e.m.) of pregnancy, whereas seven heifers, 41 ± 6 day pregnant, were given 10 mL saline (control group). Transrectal B-mode examinations of the CL were done at −1, 3, 6, 12, 24, 48, 72 and 96 h relative to treatment. Blood samples were collected at −1, 0.5, 1, 2, 3, 4, 6, 9, 12, 24, 48, 72 and 96 h. At 12 and 48 h, the CL was biopsied. None of the cows still in the experiment 10 day after LPS (n = 7) had embryonic loss. In LPS-treated cows, luteal area decreased (from 4.1 to 3.1 cm2; P ≤ 0.05) within 6 h and until 48 h. Luteal blood flow decreased by 39% (P ≤ 0.05) within the first 6 h after LPS, but returned to pre-treatment values by 48 h. Plasma P4 decreased by 62% (P ≤ 0.05), reached a nadir (2.7 ± 0.6 ng/mL) at 12 h after LPS and was not restored to pre-treatment (P ≤ 0.05). In luteal tissue, mRNAs for STAR and for FGF1 were lower (P ≤ 0.05) in LPS than in saline-treated cattle at 12 h, with no difference between groups at 48 h. Levels of mRNAs for CASP3 and FGF2 were not different between groups (P > 0.05) at 12 or 48 h after treatment. In conclusion, LPS transiently suppressed CL function, but did not induce embryonic mortality.

 

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    Median ± MAD of luteal tissue area (panel A) and mean ± s.e.m. of luteal blood flow (panel B) in early pregnant cattle treated with E. coli lipopolysaccharide (LPS; −1 to 12 h: n = 8, 24–96 h: n = 7) or Saline (n = 7), respectively. *Values differed between LPS- and Saline-treated cattle (P ≤ 0.05). a,bWithin LPS-treated cattle, values without a common superscript differed (P ≤ 0.05).

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    Median ± MAD of serum progesterone (panel A) and PGFM concentration (panel B) in early pregnant cattle treated with E. coli lipopolysaccharide (LPS;−1 to 12 h: n = 8, 24–96 h: n = 7) or Saline (n = 7), respectively. *Values differed between LPS- and Saline-treated cattle (P ≤ 0.05). a,b,A,BWithin LPS-treated cattle, values without a common superscript differed (P ≤ 0.05).

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    Mean ± s.e.m. levels of mRNA for STAR (panel A), CASP3 (panel B), FGF1 (panel C) and FGF2 (panel D) 12 and 48 h after infusion of E. coli lipopolysaccharide (LPS; dark bars) or Saline (NaCl; gray bars) in early pregnant cattle. *Values differed between LPS- and Saline-treated cattle within a sampling period (i.e. 12 or 48 h) (P ≤ 0.05). a,bWithin LPS-treated cattle, values without a common superscript differed (P ≤ 0.05).

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