Derivation of sheep embryonic stem cells under optimized conditions

in Reproduction
Authors:
Marcela Vilarino Department of Animal Science, University of California Davis, Davis, California, USA

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Delia Alba Soto Department of Animal Science, University of California Davis, Davis, California, USA

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Yanina Soledad Bogliotti Department of Animal Science, University of California Davis, Davis, California, USA

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Leqian Yu Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA

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Yanli Zhang Department of Animal Science, University of California Davis, Davis, California, USA

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Chunsheng Wang Department of Animal Science, University of California Davis, Davis, California, USA

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Erika Paulson Department of Animal Science, University of California Davis, Davis, California, USA

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Cuiqing Zhong Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA

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Miaohan Jin Department of Animal Science, University of California Davis, Davis, California, USA

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Juan Carlos Izpisua Belmonte Department of Animal Science, University of California Davis, Davis, California, USA

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Jun Wu Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA

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Pablo Juan Ross Department of Animal Science, University of California Davis, Davis, California, USA

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https://orcid.org/0000-0002-3972-3754

Correspondence should be addressed to J Wu or P J Ross; Email: Jun2.Wu@utsouthwestern.edu or pross@ucdavis.edu
DOI:
https://doi.org/10.1530/REP-19-0606
Volume/Issue:
Volume 160: Issue 5
Page Range:
761–772
Article Type:
Research Article
Online Publication Date:
Nov 2020
Copyright:
© 2020 Society for Reproduction and Fertility 2020
Restricted access
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Until recently, it has been difficult to derive and maintain stable embryonic stem cells lines from livestock species. Sheep ESCs with characteristics similar to those described for rodents and primates have not been produced. We report the derivation of sheep ESCs under a chemically defined culture system containing fibroblast growth factor 2 (FGF2) and a tankyrase/Wnt inhibitor (IWR1). We also show that several culture conditions used for stabilizing naïve and intermediate pluripotency states in humans and mice were unsuitable to maintain ovine pluripotency in vitro. Sheep ESCs display a smooth dome-shaped colony morphology, and maintain an euploid karyotype and stable expression of pluripotency markers after more than 40 passages. We further demonstrate that IWR1 and FGF2 are essential for the maintenance of an undifferentiated state in de novo derived sheep ESCs. The derivation of stable pluripotent cell lines from sheep blastocysts represents a step forward toward understanding pluripotency regulation in livestock species and developing novel biomedical and agricultural applications.

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Full Text Views 2977 124 19
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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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