Aneuploidy during the onset of mouse embryo development

in Reproduction
Authors:
Tereza Pauerova Department of Genetics and Reproduction, Central European Institute of Technology, Veterinary Research Institute, Brno, Czech Republic
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic

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Lenka Radonova Department of Genetics and Reproduction, Central European Institute of Technology, Veterinary Research Institute, Brno, Czech Republic
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic

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Kristina Kovacovicova Department of Genetics and Reproduction, Central European Institute of Technology, Veterinary Research Institute, Brno, Czech Republic

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Lucia Novakova Department of Genetics and Reproduction, Central European Institute of Technology, Veterinary Research Institute, Brno, Czech Republic
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic

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Michal Skultety Department of Genetics and Reproduction, Central European Institute of Technology, Veterinary Research Institute, Brno, Czech Republic
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic

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Martin Anger Department of Genetics and Reproduction, Central European Institute of Technology, Veterinary Research Institute, Brno, Czech Republic
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libechov, Czech Republic

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https://orcid.org/0000-0003-0128-1054

Correspondence should be addressed to M Anger; Email: anger@vri.cz
DOI:
https://doi.org/10.1530/REP-20-0086
Volume/Issue:
Volume 160: Issue 5
Page Range:
773–782
Article Type:
Research Article
Online Publication Date:
Nov 2020
Copyright:
© 2020 Society for Reproduction and Fertility 2020
Restricted access
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Aneuploidy is the most frequent single cause leading into the termination of early development in human and animal reproduction. Although the mouse is frequently used as a model organism for studying the aneuploidy, we have only incomplete information about the frequency of numerical chromosomal aberrations throughout development, usually limited to a particular stage or assumed from the occurrence of micronuclei. In our study, we systematically scored aneuploidy in in vivo mouse embryos, from zygotes up to 16-cell stage, using kinetochore counting assay. We show here that the frequency of aneuploidy per blastomere remains relatively similar from zygotes until 8-cell embryos and then increases in 16-cell embryos. Due to the accumulation of blastomeres, aneuploidy per embryo increases gradually during this developmental period. Our data also revealed that the aneuploidy from zygotes and 2-cell embryos does not propagate further into later developmental stages, suggesting that embryos suffering from aneuploidy are eliminated at this stage. Experiments with reconstituted live embryos revealed, that hyperploid blastomeres survive early development, although they exhibit slower cell cycle progression and suffer frequently from DNA fragmentation and cell cycle arrest.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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