Hormone-responsive organoids from domestic mare and endangered Przewalski’s horse endometrium

in Reproduction
Authors:
Riley E Thompson Department of Large Animal Clinical Sciences, University of Tennessee, Knoxville, Tennessee, USA
Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, Virginia, USA

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Aime K Johnson Department of Clinical Sciences, Auburn University, Auburn, Alabama, USA

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Pouya Dini Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA

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Margherita Y Turco Centre for Trophoblast Research, University of Cambridge, Cambridge, UK

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Tulio M Prado Department of Large Animal Clinical Sciences, University of Tennessee, Knoxville, Tennessee, USA

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Christopher Premanandan Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, USA

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Graham J Burton Centre for Trophoblast Research, University of Cambridge, Cambridge, UK

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https://orcid.org/0000-0001-8677-4143
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Barry A Ball Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA

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https://orcid.org/0000-0002-0502-0276
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Brian K Whitlock Department of Large Animal Clinical Sciences, University of Tennessee, Knoxville, Tennessee, USA

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Budhan S Pukazhenthi Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, Virginia, USA

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Correspondence should be addressed to B S Pukazhenthi; Email: pukazhenthib@si.edu
DOI:
https://doi.org/10.1530/REP-20-0266
Volume/Issue:
Volume 160: Issue 6
Page Range:
819–831
Article Type:
Research Article
Online Publication Date:
Dec 2020
Copyright:
© 2020 Society for Reproduction and Fertility 2020
Restricted access
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The endometrium, the inner uterine lining, is composed of cell layers that come in direct contact with an embryo during early pregnancy and later with the fetal placenta. The endometrium is responsible for signals associated with normal reproductive cyclicity as well as maintenance of pregnancy. In the mare, functionally competent in vitro models of the endometrium have not been successful. Furthermore, the ability to study various reproductive processes in vitro may allow critical evaluation of signaling pathways involved in the reproductive diseases of animals that cannot be handled frequently, such as various wildlife species. Here we report the establishment of organoids, 3D structures, derived from fresh and frozen–thawed equine endometrium (Equus ferus caballus and E. f. przewalskii). Although organoids from domestic mares responded to exogenous hormonal stimuli, organoids from Przewalski’s horse failed to respond to exogenous hormones. The present study represents a ‘first’ for any large animal model or endangered species. These physiologically functional organoids may facilitate improved understanding of normal reproductive mechanisms, uterine pathologies, and signaling mechanisms between the conceptus and endometrium and may lead to the development of novel bioassays for drug discovery.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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