SOCE-inhibitor reduced human sperm-induced formation of neutrophil extracellular traps

in Reproduction
Authors:
Fabiola Zambrano Center of Excellence in Translational Medicine-Scientific and Technological Bioresource Nucleus (CEMT – BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile
Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

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Liliana Silva Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany

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Pamela Uribe Center of Excellence in Translational Medicine-Scientific and Technological Bioresource Nucleus (CEMT – BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile
Department of Internal Medicine, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

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Ulrich Gärtner Institute of Anatomy and Cell Biology, Justus Liebig University Giessen, Giessen, Germany

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Anja Taubert Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany

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Mabel Schulz Center of Excellence in Translational Medicine-Scientific and Technological Bioresource Nucleus (CEMT – BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile
Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

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Raúl Sánchez Center of Excellence in Translational Medicine-Scientific and Technological Bioresource Nucleus (CEMT – BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile
Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

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Carlos Hermosilla Institute of Parasitology, Faculty of Veterinary Medicine, Justus Liebig University Giessen, Giessen, Germany

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Correspondence should be addressed to R Sanchez; Email: raul.sanchez@ufrontera.cl
DOI:
https://doi.org/10.1530/REP-20-0185
Volume/Issue:
Volume 161: Issue 1
Page Range:
21–29
Article Type:
Research Article
Online Publication Date:
Jan 2021
Copyright:
© 2021 Society for Reproduction and Fertility 2021
Restricted access
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Human spermatozoa activate neutrophil extracellular traps (NETs) in vitro. NETosis is an efficient mechanism through which polymorphonuclear neutrophils (PMN) capture sperm in vitro. The objective of this study was to establish the role of store-operated Ca+2 entry (SOCE) in human sperm-triggered NETs and its impact on sperm integrity and oocyte binding capacity. PMN isolated from donors were exposed to spermatozoa isolated from normozoospermic donors using the swim-up technique and were divided into the following groups: (1) sperm, (2) PMN, (3) PMN + sperm, (4) PMN (pretreated with 2-APB, SOCE inhibitor) + sperm, (5) (PMN + DNase) + sperm, and (6) (PMN + PMA) + sperm (positive control). NETs were quantified using PicoGreen® and visualised by scanning electron microscopy and immunofluorescence of extracellular DNA and neutrophil elastase. Plasma membrane, acrosome, and DNA integrity were analysed by flow cytometry, and oocyte binding was evaluated using the hemizona pellucida assay. Sperm-triggered NETosis negatively affected the sperm membrane and acrosome integrity and decreased the oocyte binding capacity. These effects were negated by an SOCE inhibitor, thus improving sperm function and achieving high oocyte binding capacity. The SOCE inhibitor significantly reduced NET formation compared with that in control PMN/sperm (P < 0.05). Collectively, these results advance the knowledge about the role of PMN in reproduction and will allow the development of strategies to block NET formation in situations of reduced fertilisation success.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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