Avian sperm increase in vitro the release of exosomes from SST-enriched organoids

in Reproduction
Authors:
Luiz Cordeiro CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France
Federal University of Semi Arid Region, Mossoro, Rio Grande do Norte, Brazil

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Cindy Riou CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France
ALLICE, Station de Phénotypage, Lieu-Dit Le Perroi, Nouzilly, France

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Rustem Uzbekov Laboratoire Biologie Cellulaire, Microscopie Electronique, Faculté de Médecine, Université de Tours, Tours, France
Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia

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Nadine Gérard CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France

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Correspondence should be addressed to N Gérard; Email: nadine.gerard@inrae.fr
DOI:
https://doi.org/10.1530/REP-20-0421
Volume/Issue:
Volume 161: Issue 4
Page Range:
375–384
Article Type:
Research Article
Online Publication Date:
Apr 2021
Copyright:
© 2021 Society for Reproduction and Fertility 2021
Restricted access
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In birds, oviductal cells play a crucial role in the storage of sperm via cell-to-cell communication including extracellular vesicles (EV). We developed a culture of oviductal organoids enriched in sperm storage tubules (SSTorg) to demonstrate the release of EV. SSTorg were cultured for 24 h and added to live (LV), frozen (FZ) and lysed (LY) avian sperm, seminal plasma (SP), avian sperm conditioned medium (CM), or bovine sperm (BV). Western blot demonstrated that SSTorg contained EV protein markers, valosin-containing protein (VCP), heat shock proteins (HSP90AA1, HSPA8), and annexins (ANXA2, A4, A5). Co-culture with LV significantly decreased the intracellular level of all these proteins except HSPA8. Immunohistochemistry confirmed this result for VCP and ANXA4. LY, CM, SP and BV had no effect on the intracellular level of these proteins, whereas FZ induced a decrease in ANXA2, A4 and A5. In culture media, VCP and HSP90AA1 signals were detected in the presence of LV, FZ, BV, LY, CM and SP, but no ANXA4 signal was observed in the presence of FZ and SP. ANXA2 and A5 were only detected in the presence of LV. The most abundant EV were less than 150 nm in diameter. ANXA4 and A5 were more abundant in EV isolated from the SSTorg culture medium. This study provides a useful culture system for studying interactions between SST cells and sperm. We demonstrated the release of EV by SSTorg in vitro, and its regulation by sperm. This may be of crucial importance for sperm during storage in hens.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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