bta-miR-183 targets EZRIN to regulate microvilli formation and improve early development of bovine embryos

in Reproduction
Authors:
Yanfang Wu College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Zhenzi Zuo State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing, P.R. China

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Zheng Wang College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Hanghang Liu College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Qi Zhou College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Subi Ren College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Xinrui Lan College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Yong Zhang College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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Yongsheng Wang College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, P.R. China
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, P.R. China

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https://orcid.org/0000-0002-8301-4031

Correspondence should be addressed to Y Wang; Email: wangyongsheng01@nwsuaf.edu.cn

*(Y Wu, Z Zuo and Z Wang contributed equally to this work)

DOI:
https://doi.org/10.1530/REP-22-0361
Volume/Issue:
Volume 165: Issue 4
Page Range:
363–371
Article Type:
Research Article
Online Publication Date:
08 Mar 2023
Copyright:
© Society for Reproduction and Fertility 2023
Restricted access
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In brief

Almost total lack of sperm-borne RNAs is regarded as one of the key factors that leads to the abnormal development of somatic cell nuclear transfer embryo. This paper reveals a need for us to further explore the roles of the paternal regulatory factors on embryonic development in early embryos.

Abstract

Mature sperm contain both coding and non-coding RNAs, which can be delivered into an oocyte with the sperm at fertilization. Accumulating evidences show that these sperm-borne RNAs play crucial roles in epigenetic reprogramming, cytoskeleton remodeling, embryonic development, and offspring phenotype. Almost total lack of sperm-borne RNAs is regarded as one of the key factors that leads to the abnormal development of somatic cell nuclear transfer (SCNT) embryo. bta-miR-183 was found to be highly expressed in bovine sperm and can be delivered into oocytes during fertilization in our previous study, and in this study, EZR was confirmed as a target gene of bta-miR-183 in early embryos by bioinformatics, luciferase, and gain-of-function and loss-of-function experiments. Scanning electron microscopy showed that the density of microvilli on the surface of SCNT embryos was significantly higher than that onin vitro fertilized embryos and was significantly reduced by injection of bta-miR-183 mimic. EZR-siRNA injected into SCNT embryos had a similar effect. This indicated that the lack of bta-miR-183 might lead to abnormal changes in microvilli by downregulating ezrin protein. In addition, gain-of-function studies showed that bta-miR-183 significantly improved developmental competence of SCNT embryo in terms of cleavage (76.63% vs 64.32%, P < 0.05), blastocyst formation (43.75% vs 28.26%, P < 0.05), apoptotic index (5.21% vs 12.64%, P < 0.05), and the trophoblast ratio (32.65% vs 25.58%, P < 0.05) in day 7 blastocysts. Thus, the present study indicated that bta-miR-183 might play crucial roles in the formation of microvilli and embryo development by regulating expression of EZR mRNA.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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