Exosomal or follicular FNDC3A decreases FOLR1 mRNA abundance and progesterone and lactate synthesis in bovine granulosa cells

in Reproduction
Authors:
Mathilde Daudon CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France
Centre de recherche en reproduction et fertilité, Faculté de médecine vétérinaire, Université de Montréal, Saint Hyacinthe, Quebec, Canada

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https://orcid.org/0000-0001-9998-0421
,
Christelle Ramé CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France

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,
Christopher A Price Centre de recherche en reproduction et fertilité, Faculté de médecine vétérinaire, Université de Montréal, Saint Hyacinthe, Quebec, Canada

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https://orcid.org/0000-0002-6727-8280
, and
Joëlle Dupont CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly, France

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https://orcid.org/0000-0002-8046-7705

Correspondence should be addressed to C A Price or J Dupont; Email: christopher.price@umontreal.ca or joelle.dupont@inrae.fr

*(C A Price and J Dupont contributed equally to this work as senior authors)

DOI:
https://doi.org/10.1530/REP-23-0451
Volume/Issue:
Volume 167: Issue 5
Article ID:
e230451
Article Type:
Research Article
Online Publication Date:
17 Apr 2024
Copyright:
© the author(s) 2024
Restricted access
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In brief

Dairy cattle experience a period of infertility postpartum that is caused in part by the development of IGF1/insulin resistance. This study suggests that an adipokine, FNDC3A, reduces IGF1-dependent glycolysis and may contribute to postpartum infertility.

Abstract

Dairy cows go through a period of subfertility after parturition, triggered in part by a disruption of energy homeostasis. The mobilization of body fat alters the secretion of adipokines, which have been shown to impact ovarian function. Fibronectin type III domain-containing 3A (FNDC3A) is a recently discovered adipokine-myokine, and FNDC3A mRNA abundance in subcutaneous adipose tissue is increased postpartum in cattle. In this study, we hypothesized that FNDC3A may compromise granulosa cell function in cattle and investigated this using a well-established in vitro cell culture model. Here, we demonstrate the presence of FNDC3A protein associated with extracellular vesicles in follicular fluid and in plasma, suggesting an endocrine role for this adipokine. FNDC3A protein and mRNA was also detected in the bovine ovary (cortex, granulosa and theca cells, cumulus, oocyte and corpus luteum). Abundance of FNDC3A mRNA in granulosa cells from small follicles was increased by in vitro treatment with the adipokines leptin and TNF but not by visfatin, resistin, adiponectin, chemerin or IGF1. Addition of recombinant FNDC3A at physiological doses (10 ng/mL) to granulosa cells decreased IGF1-dependent progesterone but not estradiol secretion and IGF1-dependent lactate secretion and abundance of GLUT3 and GLUT4 mRNA. This concentration of FNDC3A increased cell viability, abundance of mRNA encoding a putative receptor FOLR1, and increased phosphorylation of Akt. Collectively, these data suggest that FNDC3A may regulate folliculogenesis in cattle by modulating IGF1-dependent granulosa cell steroidogenesis and glucose metabolism.

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Sept 2018 onwards Past Year Past 30 Days
Full Text Views 144 144 8
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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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