The effects of the activation of TLR2/TLR1 on in vitro angiogenesis in an immortalized ovine luteal endothelial cell line

in Reproduction
Authors:
Duygu Yaman Gram Department of Physiology, Faculty of Veterinary Medicine, Erciyes University, Turkey

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Murat Abay Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Erciyes University, Turkey

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Narin Liman Department of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes University, Turkey

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Muhittin Tekin Clinical Unit for Herd Health Management in Ruminants, Center for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, Austria

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Mariusz P Kowalewski Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland

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https://orcid.org/0000-0002-4565-7714
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Aykut Gram Department of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes University, Turkey

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https://orcid.org/0000-0002-7490-5836

Correspondence should be addressed to A Gram: aykutgram@erciyes.edu.tr
DOI:
https://doi.org/10.1530/REP-23-0368
Volume/Issue:
Volume 168: Issue 4
Article ID:
e230368
Article Type:
Research Article
Online Publication Date:
11 Sep 2024
Copyright:
© the author(s) 2024
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In brief

Activation of TLR2/TLR1 alters in vitro formation of capillary-like structures and induces inflammatory processes in ovine luteal endothelial (OLENDO) cells.

Abstract

Postpartum bacterial infections of the uterus affect uterine physiology and ovarian activity, causing fertility problems. The outer membrane component of Gram-negative bacteria, lipopolysaccharide, is involved in the initiation of the local inflammatory processes, and other bacterial toxins, particularly lipopeptides, have also been shown to be potent cytokine inducers, acting via Toll-like receptor-2 (TLR2). However, the possible adverse effects of TLR2 on ovarian and luteal activities have not yet been investigated in depth. The strong expression of TLR2 in the blood vessels of the corpus luteum led us to hypothesize that TLR2 activation might participate in the disruption of luteal vascular functionality. Therefore, we analyzed the effects of Pam3CSK4 (Pam3CysSerLys4), a synthetic triacylated lipopeptide and TLR2/TLR1 ligand, on the functionality of gap junctional intercellular communication (GJIC), endothelial cell invasion, and in vitro capillary-like network formation in an immortalized ovine luteal endothelial (OLENDO) cell line. Pam3CSK4 treatment of OLENDO cells disrupted in vitro tube formation but had no effect on GJIC or migration of OLENDO cells. Furthermore, Pam3CSK4 induced the expression of NFKB, IL6, and IL8 in OLENDO cells. Additionally, the basal availability of TLRs (TLR1–10) and TLR co-receptors (MYD88, LY96/MD2, and CD14) in OLENDO cells was confirmed by conventional PCR. Finally, the activation of TLR2/TLR1 appears to alter in vitro formation of capillary-like structures and induce inflammatory processes in OLENDO cells.

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Print ISSN:
1470-1626
Online ISSN:
1741-7899

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