Haemolytic factor, the protein responsible for the haemolysis of red blood cells, was isolated from bull seminal vesicle fluid. The purified protein has a mol. wt of 80,000. Haemolytic factor dissociates in acidic urea buffer into sub-units with a mol. wt approximately ten times lower. Easy transitions in the sub-unit arrangement observed under various conditions are probably the reason for changes in electrophoretic mobility and in haemolytic activity.
If the inline PDF is not rendering correctly, you can download the PDF file here.