THE LABELLING OF LIVING RABBIT OVA WITH FLUORESCENT DYES

in Reproduction

Summary.

Rabbit ova can be labelled with fluorescein isothiocyanate (FITC) or rhodamine blue isothiocyanate (RBITC) for use in mixed egg-transfer experiments. Both FITC and RBITC bind to the zona pellucida of the treated egg, allowing recognition of experimental and control groups in a mixed population. Labelling ova with one of these dyes is not detrimental to their penetrability or fertilizability and can be of significant value in a fertilization assay in which experimental and control ova are transferred to the same tubal environment.

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    Society for Reproduction and Fertility

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