Inhibition of ovulation in PMSG/hCG-treated immature rats by rotenone, a specific inhibitor of mitochondrial oxidation

in Reproduction

Summary. Immature Wistar rats were induced to ovulate by treatment with PMSG and hCG. Control animals ovulated 43·5 ± 0·36 ova/rat. Intraperitoneal injection of rotenone doses of 0·125, 0·25 and 0·50 mg/kg reduced the ovulation rate to 24·0 ± 3·08, 8·0 ± 0·88 and 1·5 ± 0·44 ova/rat, respectively. The rotenone significantly reduced ovarian cytochrome oxidase activity and progesterone production, but not production of oestradiol or testosterone. Thyroxine treatment at a dose of 5 mg/kg s.c. reversed the rotenone inhibition of ovulation. The results suggest that an increase in mitochondrial respiration is an essential feature of the ovulation process in mammals.

If the inline PDF is not rendering correctly, you can download the PDF file here.


     An official journal of

    Society for Reproduction and Fertility


Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 73 19 1
PDF Downloads 94 23 2