Immunological aspects of follicle ovulation and corpus luteum formation in cattle

in Reproduction
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  • 1 N Abdulrahman Alrabiah, School of Agriculture and Food Science, University College Dublin, Dublin, Ireland
  • 2 A Evans, School of Agriculture and Food Science, University College Dublin, Dublin, Ireland
  • 3 A Fahey, Agriculture and Food Science, University College Dublin, Dublin, 4, Ireland
  • 4 N Cantwell, School of Agriculture and Food Science, University College Dublin, Dublin, Ireland
  • 5 P Lonergan, School of Agriculture and Food Science, University College Dublin, Dublin, Ireland
  • 6 J McCormack, Research Pathology Core Facility, Conway Institute, University College Dublin, Dublin, Ireland
  • 7 J Browne, School of Agriculture and Food Science, University College Dublin, Dublin, Ireland
  • 8 T Fair, School of Agriculture and Food Science, University College Dublin, Dublin, Ireland

Correspondence: Trudee Fair, Email: trudee.fair@ucd.ie
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Ovulation has been described as an inflammatory event, characterized by an influx of leukocytes into the ovulatory follicle and changes in the expression of immune factors in both the theca and granulosa tissue layers. Since information on this process is limited in cattle, our objective was to elucidate the contribution of the immune system to dominant follicle luteinization, ovulation and corpus luteum formation in cattle. Beef heifers (n=50) were oestrous synchronized, slaughtered and ovarian follicular or luteal tissue collected during a 96h window around ovulation. Follicular fluid cytokine concentration, temporal immune cell infiltration and inflammatory status were determined by Luminex multiplex analysis, immunohistochemistry and quantitative real time PCR-analysis, respectively, in pre- and peri-ovulatory follicular tissues. The concentrations of CXCL10 and VEGF-A were highest in pre-ovulatory follicular fluid samples. The pre and peri -ovulatory follicles play host to a broad repertoire of immune cells, including T-cells, granulocytes and monocytes. Dendritic cells were the most abundant cells in ovulatory follicular and luteal -tissue at all times. The mRNA expression of candidate genes associated with inflammation was highest in pre- and peri-ovulatory tissue, whereas tissue growth and modelling factors were highest in the post-ovulatory follicular and early luteal tissue. In conclusion, ovulation in cattle is characterized by the presence of neutrophils, macrophages and dendritic cells in the ovulatory follicle, reflected in compartmentalized cytokine and growth factor expression. These findings indicate a tightly regulated sterile inflammatory response to the LH surge in the ovulatory follicle which is rapidly resolved during early corpus luteum formation.

 

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