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A. G. SACCO and C. A. SHIVERS

Summary.

The indirect fluorescent antibody technique was used to localize the reproductive tissue-specific antigens present in the rabbit ovary, oviduct and uterus. The two ovarian antigens were localized in the zona pellucida, theca interna and atretic follicles; the six oviducal antigens in the epithelium of the mucosa; and the two uterine antigens in the endometrial stroma and lumina of uterine glands and the lamina propria of the cervix.

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A. G. SACCO and C. A. SHIVERS

Summary.

Sheep antisera were prepared against saline extracts of rabbit ovary, oviduct and uterus. The Ouchterlony double diffusion technique was employed to detect the antigens specific to the reproductive tissues in these organs. The unabsorbed antisera produced the following numbers of precipitin bands: anti-ovary versus ovary, fifteen bands; anti-oviduct versus oviduct, twelve bands; anti-uterus versus uterus, ten bands. Absorption of each of the three antisera by rabbit plasma, kidney and spleen was necessary to detect antibodies directed against antigens specific to the reproductive tissues. The following numbers of precipitin bands were observed when such absorbed antisera were allowed to react against the homologous tissue: anti-ovary versus ovary, two bands; anti-oviduct versus oviduct, six bands; anti-uterus versus uterus, two bands. The absorbed antisera produced no bands against any of the seventeen non-reproductive tissues or testis extract. Based on the sensitivity of the Ouchterlony technique and the number of non-reproductive tissues examined, these results were interpreted as indicating that the rabbit reproductive system contains tissue-specific antigens. In the ovary, the tissue-specific antigens were restricted to that structure. Several of the tissue-specific antigens detected in oviduct and uterus, however, were also present in the other reproductive tissues.

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A. G. SACCO and C. A. SHIVERS

Summary.

Sheep antisera prepared against saline extracts of rabbit (Californian) ovary, oviduct and uterus were used to detect the presence of homologous antigens in the ovaries and reproductive tracts of the Dutch rabbit, New Zealand White (NZW) rabbit, guinea-pig, hamster, mouse and rat. Absorption of each antiserum with Californian rabbit plasma, kidney and spleen and the reaction of the absorbed antisera in immunodiffusion plates against the homologous antigen from each species produced the following results: (1) absorbed anti-ovary serum formed precipitin bands with two antigens in the ovaries of each of the breeds of rabbit while forming no bands when allowed to react with the ovaries of the other mammals tested; (2) absorbed anti-oviduct serum formed bands with six antigens in the oviducts of each of the rabbit breeds, two antigens in the mouse oviduct, one in the rat oviduct and formed no bands with the oviducal material from the guinea-pig and hamster; (3) absorbed anti-uterus serum formed bands with two antigens in the uterus of the NZW rabbit and one band with the uterine material from the Dutch rabbit and other species. None of the three absorbed antisera reacted with the sixteen non-reproductive tissues or testis preparations from each of the different species. These results demonstrated that antigens present in reproductive tissues of the Californian rabbit were also present in several other mammalian species.

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C. A. SHIVERS, C. B. METZ and C. LUTWAK-MANN

Summary.

Comparisons of the antigenic and chemical composition of pig blood and follicular fluid are reported. Follicular fluid was found to contain less protein, protein-bound orcinol-reactive carbohydrate, reducing sugar and sialic acid, but a little more acid-soluble phosphate, than blood serum. Fructose and ergothioneine were not present in appreciable amounts in either follicular fluid or blood serum

Immunoelectrophoretic analysis showed that both follicular fluid and blood serum contained at least ten different antigenic components. Most of these were common to both but two of the serum antigens were not found in the follicular fluid. The follicular fluid antigen absent from serum appears to be fibrinogen.

Washings from the uteri of adult non-pregnant pigs contained at least six antigens most of which were not found in either follicular fluid or blood.