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  • Author: M. BOBROW x
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It has been previously reported that the distal half of the long arm of the human Y chromosome shows a differential affinity for fluorescent acridine derivatives (Zech, 1969; Pearson, Bobrow & Vosa, 1970). By the use of this staining reaction, the Y chromosome can be detected in interphase nuclei of lymphocytes, cultured skin fibroblasts and buccal mucosal cells. This technique has also been used to demonstrate that the X chromosome pairs with the short arm of the Y in first meiotic prophase (Pearson & Bobrow, unpublished observation). This paper describes the appearance of the Y chromosome in the different stages of spermatogenesis. Cytological preparations for the examination of meiotic cells were prepared from testicular tissue of three normal adult males by the method of Evans, Brecon & Ford (1964). Some material was prepared without hypotonic treatment, particularly