Search Results
You are looking at 1 - 1 of 1 items for
- Author: Bin Ling x
- Refine by access: Open Access content only x
Search for other papers by Qing Li in
Google Scholar
PubMed
Search for other papers by Juncen Guo in
Google Scholar
PubMed
Search for other papers by Gelin Huang in
Google Scholar
PubMed
Search for other papers by Nan Wu in
Google Scholar
PubMed
Search for other papers by Su Chen in
Google Scholar
PubMed
Search for other papers by Jing Dai in
Google Scholar
PubMed
Search for other papers by Xueguang Zhang in
Google Scholar
PubMed
Search for other papers by Guohui Zhang in
Google Scholar
PubMed
Search for other papers by Weiwei Zhi in
Google Scholar
PubMed
Search for other papers by Jierui Yan in
Google Scholar
PubMed
Search for other papers by Rui Zheng in
Google Scholar
PubMed
Search for other papers by Fei Yan in
Google Scholar
PubMed
Search for other papers by Zheng Yan in
Google Scholar
PubMed
Search for other papers by Ling Wu in
Google Scholar
PubMed
Search for other papers by Sixian Wu in
Google Scholar
PubMed
Search for other papers by Zhiliang Ji in
Google Scholar
PubMed
Search for other papers by Jiuzhi Zeng in
Google Scholar
PubMed
Search for other papers by Ge Lin in
Google Scholar
PubMed
Search for other papers by Bin Li in
Google Scholar
PubMed
Search for other papers by Wenming Xu in
Google Scholar
PubMed
In brief
PLCZ1 mutations are related to total fertilisation failure (TFF) after intracytoplasmic sperm injection (ICSI), characterised by abnormal oocyte oscillations. The novel PLCZ1 compound heterozygous mutations reported by this study were associated with TFF after ICSI, with one of the mutations indicating a gene dosage effect.
Abstract
Oocyte activation failure is thought to be one of the main factors for total fertilisation failure (TFF) after intracytoplasmic sperm injection (ICSI), which could be induced by abnormal calcium oscillations. Phospholipase C zeta (PLCZ), a sperm factor, is associated with Ca2+ oscillations in mammalian oocytes. To date, some mutations in PLCZ1 (the gene that encodes PLCZ) have been linked to TFF, as demonstrated by the observed reduction in protein levels or activity to induce Ca2+ oscillations. In this study, normozoospermic males whose sperms exhibited TFF after ICSI and their families were recruited. First, mutations in the PLCZ1 sequence were identified by whole exome sequencing and validated using Sanger sequencing. Then, the locations of PLCZ1/PLCZ and the transcript and protein levels in the sperm of the patients were studied. Subsequently, in vitro function analysis and in silico analysis were performed to investigate the function–structure correlation of mutations identified in PLCZ1 using western blotting, immunofluorescence, RT-qPCR, and molecular simulation. Ca2+ oscillations were detected after cRNA microinjection into MII mouse oocytes to investigate calcium oscillations induced by abnormal PLCZ. Five variants with compound heterozygosity were identified, consisting of five new mutations and three previously reported mutations distributed across the main domains of PLCZ, except the EF hands domain. The transcript and protein levels decreased to varying degrees among all detected mutations in PLCZ1 when transfected in HEK293T cells. Among these, mutations in M138V and R391* of PLCZ were unable to trigger typical Ca2+ oscillations. In case 5, aberrant localisation of PLCZ in the sperm head and an increased expression of PLCZ in the sperm were observed. In conclusion, this study enhances the potential for genetic diagnosis of TFF in clinics and elucidates the possible relationship between the function and structure of PLCZ in novel mutations.
