Search Results
You are looking at 1 - 5 of 5 items for
- Author: Qi Zhou x
- Refine by access: Content accessible to me x
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China
Search for other papers by Xiangpeng Dai in
Google Scholar
PubMed
State Key Laboratory of Reproductive Biology, Graduate School of the Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China
Search for other papers by Jie Hao in
Google Scholar
PubMed
Search for other papers by Qi Zhou in
Google Scholar
PubMed
Many strategies have been established to improve the efficiency of somatic cell nuclear transfer (SCNT), but relatively few focused on improving culture conditions. The effect of different culture media on preimplantation development of mouse nuclear transfer embryos was investigated. A modified sequential media method, named D media (M16/KSOM and CZB-EG/KSOM), was successfully established that significantly improves SCNT embryo development. Our result demonstrated that while lacking any adverse effect on in vivo fertilized embryos, the D media dramatically improves the blastocyst development of SCNT embryos compared with other commonly used media, including KSOM, M16, CZB, and αMEM. Specifically, the rate of blastocyst formation was 62.3% for D1 (M16/KSOM) versus 10–30% for the other media. An analysis of media components indicated that removing EDTA and glutamine from the media can be beneficial for early SCNT embryo development. Our results suggest that in vitro culture environment plays an important role in somatic cell reprogramming, and D media represent the most efficient culture method reported to date to support mouse SCNT early embryo development in vitro.
State Key Laboratory of Reproductive Biology, Graduate School, Department of Obstetrics and Gynecology, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, People's Republic of China
Search for other papers by Liang Wu in
Google Scholar
PubMed
Search for other papers by Honghui Zhou in
Google Scholar
PubMed
Search for other papers by Haiyan Lin in
Google Scholar
PubMed
Search for other papers by Jianguo Qi in
Google Scholar
PubMed
Search for other papers by Cheng Zhu in
Google Scholar
PubMed
Search for other papers by Zhiying Gao in
Google Scholar
PubMed
Search for other papers by Hongmei Wang in
Google Scholar
PubMed
Until recently, the molecular pathogenesis of preeclampsia (PE) remained largely unknown. Reports have shown that circulating microRNAs (miRNAs) are promising novel biomarkers for cancer, pregnancy, tissue injury, and other conditions. The objective of this study was to identify differentially expressed miRNAs in plasma from severe preeclamptic pregnancies compared with plasma from normal pregnancies. By mature miRNA microarray analysis, 15 miRNAs, including 13 up- and two downregulated miRNAs, were screened to be differentially expressed in plasma from women with severe PE (sPE). Seven miRNAs, namely miR-24, miR-26a, miR-103, miR-130b, miR-181a, miR-342-3p, and miR-574-5p, were validated to be elevated in plasma from severe preeclamptic pregnancies by real-time quantitative stem-loop RT-PCR analysis. Gene ontology and pathway enrichment analyses revealed that these miRNAs were involved in specific biological process categories (including regulation of metabolic processes, regulation of transcription, and cell cycle) and signaling pathways (including the MAP kinase signaling pathway, the transforming growth factor-β signaling pathway, and pathways in cancer metastasis). This study presents, for the first time, the differential expression profile of circulating miRNAs in sPE patients. The seven elevated circulating miRNAs may play critical roles in the pathogenesis of sPE, and one or more of them may become potential markers for diagnosing sPE.
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Cai-Xia Yang in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Zichuan Liu in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Renaud Fleurot in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Pierre Adenot in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Véronique Duranthon in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Xavier Vignon in
Google Scholar
PubMed
Search for other papers by Qi Zhou in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Jean-Paul Renard in
Google Scholar
PubMed
INRA, ENVA, State Key Laboratory of Reproductive Biology, UMR 1198 Biologie du Developpement et Reproduction, F-78350 Jouy en Josas, France
Search for other papers by Nathalie Beaujean in
Google Scholar
PubMed
To investigate the embryonic genome organization upon fertilization and somatic cell nuclear transfer (SCNT), we tracked HP1β and CENP, two well-characterized protein markers of pericentric and centromeric compartments respectively, in four types of embryos produced by rabbit in vivo fertilization, rabbit parthenogenesis, rabbit-to-rabbit, and bovine-to-rabbit SCNT. In the interphase nuclei of rabbit cultured fibroblasts, centromeres and associated pericentric heterochromatin are usually isolated. Clustering into higher-order chromatin structures, such as the chromocenters seen in mouse and bovine somatic cells, could not be observed in rabbit fibroblasts. After fertilization, centromeres and associated pericentric heterochromatin are quite dispersed in rabbit embryos. The somatic-like organization is progressively established and completed only by the 8/16-cell stage, a stage that corresponds to major embryonic genome activation in this species. In SCNT embryos, pericentric heterochromatin distribution typical for rabbit and bovine somatic cells was incompletely reverted into the 1-cell embryonic form with remnants of heterochromatin clusters in 100% of bovine-to-rabbit embryos. Subsequently, the donor cell nuclear organization was rapidly re-established by the 4-cell stage. Remarkably, the incomplete remodeling of bovine-to-rabbit 1-cell embryos was associated with delayed transcriptional activation compared with rabbit-to-rabbit embryos. Together, the results confirm that pericentric heterochromatin spatio-temporal reorganization is an important step of embryonic genome reprogramming. It also appears that genome reorganization in SCNT embryos is mainly dependent on the nuclear characteristics of the donor cells, not on the recipient cytoplasm.
Search for other papers by Yang Yu in
Google Scholar
PubMed
Search for other papers by Chenhui Ding in
Google Scholar
PubMed
Search for other papers by Eryao Wang in
Google Scholar
PubMed
Search for other papers by Xinjie Chen in
Google Scholar
PubMed
Search for other papers by Xuemei Li in
Google Scholar
PubMed
Search for other papers by Chunli Zhao in
Google Scholar
PubMed
Search for other papers by Yong Fan in
Google Scholar
PubMed
Search for other papers by Liu Wang in
Google Scholar
PubMed
Search for other papers by Nathalie Beaujean in
Google Scholar
PubMed
Search for other papers by Qi Zhou in
Google Scholar
PubMed
Search for other papers by Alice Jouneau in
Google Scholar
PubMed
Search for other papers by Weizhi Ji in
Google Scholar
PubMed
Even though it generates healthy adults, nuclear transfer in mammals remains an inefficient process. Mainly attributed to abnormal reprograming of the donor chromatin, this inefficiency may also be caused at least partly by a specific effect of the cloning technique which has not yet been well investigated. There are two main procedures for transferring nuclei into enucleated oocytes: fusion and piezoelectric microinjection, the latter being used mostly in mice. We have, therefore, decided to compare the quality and the developmental ability, both in vivo and in vitro, of embryos reconstructed with electrofusion or piezoelectric injection. In addition, the effect of piezo setups of differing electric strengths was investigated. Along with the record of the rate of development, we compared the nuclear integrity in the blastomeres during the first cleavages as well as the morphological and cellular quality of the blastocysts. Our results show that the piezo-assisted micromanipulation can induce DNA damage in the reconstructed embryos, apoptosis, and reduced cell numbers in blastocysts as well as a lower rate of development to term. Even if piezo-driven injection facilitates a faster and more efficient rate of reconstruction, it should be used with precaution and with as low parameters as possible.
Search for other papers by Da Li in
Google Scholar
PubMed
Search for other papers by Yue You in
Google Scholar
PubMed
Search for other papers by Fang-Fang Bi in
Google Scholar
PubMed
Search for other papers by Tie-Ning Zhang in
Google Scholar
PubMed
Search for other papers by Jiao Jiao in
Google Scholar
PubMed
Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut, USA
Search for other papers by Tian-Ren Wang in
Google Scholar
PubMed
Search for other papers by Yi-Ming Zhou in
Google Scholar
PubMed
Search for other papers by Zi-Qi Shen in
Google Scholar
PubMed
Search for other papers by Xiu-Xia Wang in
Google Scholar
PubMed
Search for other papers by Qing Yang in
Google Scholar
PubMed
The importance of autophagy in polycystic ovary syndrome (PCOS)-related metabolic disorders is increasingly being recognized, but few studies have investigated the role of autophagy in PCOS. Here, transmission electron microscopy demonstrated that autophagy was enhanced in the ovarian tissue from both humans and rats with PCOS. Consistent with this, ovarian granulosa cells from PCOS rats showed increases in the autophagy marker protein light chain 3B (LC3B), whereas levels of the autophagy substrate SQSTM1/p62 were decreased. In addition, the ratio of LC3-II/LC3-I was markedly elevated in human PCOS ovarian tissue compared with normal ovarian tissue. Real-time PCR arrays indicated that 7 and 34 autophagy-related genes were down- and up-regulated in human PCOS , Signal-Net, and regression analysis suggested that there are a wide range of interactions among these 41 genes, and a potential network based on EGFR, ERBB2, FOXO1, MAPK1, NFKB1, IGF1, TP53 and MAPK9 may be responsible for autophagy activation in PCOS. Systematic functional analysis of 41 differential autophagy-related genes indicated that these genes are highly involved in specific cellular processes such as response to stress and stimulus, and are linked to four significant pathways, including the insulin, ERBB, mTOR signaling pathways and protein processing in the endoplasmic reticulum. This study provides evidence for a potential role of autophagy disorders in PCOS in which autophagy may be an important molecular event in the pathogenesis of PCOS.
