The preservation of the motility and fertilizing capacity of fowl spermatozoa was evaluated under low temperature-low concentration conditions in low-ionic-strength glycine-citrate and glycine-phosphate buffers. Solutions of both buffers at 310 mosmol gave less than a 15% decrement from initial motility values after storage for 6 hr at 3° C in concentrations from 8 to 80× 106 spermatozoa/ml. Fertility results with spermatozoa diluted 1:30 in glycine-citrate buffer, stored for 1½ hr at 3° C and re-concentrated by centrifugation, did not differ from control values, using 1:2 dilution in modified Krebs' solution. These experimental media are thus proved capable of maintaining the vital functions of fowl spermatozoa during storage and for certain desired biophysical studies. They may, therefore, be of potential practical value for work in poultry husbandry and genetics.