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Keqin Yan Department of Obstetrics and Gynecology, China–Japan Friendship Hospital, Beijing, China

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Dingqing Feng Department of Obstetrics and Gynecology, China–Japan Friendship Hospital, Beijing, China

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Jing Liang Department of Obstetrics and Gynecology, China–Japan Friendship Hospital, Beijing, China

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Qing Wang Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China

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Lin Deng Department of Obstetrics and Gynecology, China–Japan Friendship Hospital, Beijing, China

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Xiao Zhang Department of Obstetrics and Gynecology, China–Japan Friendship Hospital, Beijing, China

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Bin Ling Department of Obstetrics and Gynecology, China–Japan Friendship Hospital, Beijing, China

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Daishu Han Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China

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Viral infections of the ovary may perturb ovarian functions. However, the mechanisms underlying innate immune responses in the ovary are poorly understood. The present study demonstrates that cytosolic viral DNA sensor signaling initiates the innate immune response in mouse ovarian granulosa cells and affects endocrine function. The cytosolic DNA sensors p204 and cGAS and their common signaling adaptor stimulator of interferon (IFN) genes (STING) were constitutively expressed in granulosa cells. Transfection with VACV70, a synthetic vaccinia virus (VACV) DNA analog, induced the expression of type I interferons (IFNA/B) and major inflammatory cytokines (TNFA and IL6) through IRF3 and NF-κB activation respectively. Moreover, several IFN-inducible antiviral proteins, including 2′,5′-oligoadenylate synthetase, IFN-stimulating gene 15 and Mx GTPase 1, were also induced by VACV70 transfection. The innate immune responses in granulosa cells were significantly reduced by the transfection of specific small-interfering RNAs targeting p204, cGas or Sting. Notably, the VACV70-triggered innate immune responses affected steroidogenesis in vivo and in vitro. The data presented in this study describe the mechanism underlying ovarian immune responses to viral infection.

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