Search Results

You are looking at 1 - 10 of 13 items for

  • Author: A. R. SHETH x
Clear All Modify Search
Free access

A. R. SHETH and SHANTA S. RAO

Summary.

The maltase activity of human seminal plasma was studied by paper chromatographic and glucose oxidase methods and expressed in terms of the glucose liberated from the maltose substrate used. The specificity of maltase was established by selectively inhibiting the enzyme activity with tris buffer. Seminal maltase and amylase were separated and partially purified. Purified maltase preparation did not exhibit any amylase, maltose phosphorylase or transglucosidase activity. The maltase activity of semen is predominantly associated with seminal plasma and proceeds at maximal velocity at pH 6·0. The prostate gland seems to contribute most of the enzyme activity to the seminal plasma. The significance of the presence of maltase in seminal plasma and also in cervical mucus is discussed.

Free access

Neelam Goomer, R. N. Saxena and A. R. Sheth

Summary. The hypothalamic LH-RH content and the concentrations of pituitary and plasma LH were measured at various ages in female rats treated daily with 10 μg testosterone propionate or 10 μg oestradiol-17β from birth to Day 15. Persistent vaginal oestrus was induced in all the treated rats. Both hormones significantly reduced the hypothalamic LH-RH content and pituitary and plasma LH concentrations. Hypothalamic LH-RH increased after cessation of treatment but pituitary LH did not return to normal levels. Plasma LH levels were significantly lower than those in control rats. It is concluded that testosterone propionate and oestradiol-17β (1) have a direct negative feed-back influence on the hypothalamus in the neonatal female rat; (2) alter the normal pattern of plasma and pituitary LH in developing female rats; (3) prevent the cyclic secretion of plasma LH after maturity; and (4) probably cause a chronic impairment in the release of LH-RH.

Free access

A. R. SHETH, K. P. GUNAGA and SHANTA S. RAO

Summary.

The amylo-1,6-glucosidase activity of human seminal plasma was studied using its specific substrate, α-glucosyl Schardinger dextrin. The enzyme activity of semen is predominantly associated with the seminal plasma and proceeds at maximum velocity at pH 5·8 to 6·0. The secretions of seminal vesicles and testis seem to contribute most of the enzyme activity to the seminal plasma. Amylo-1,6-glucosidase activity is significantly lower in azoospermic samples as compared to those with a good sperm count.

Free access

K. P. GUNAGA, A. R. SHETH and SHANTA S. RAO

Summary.

Testes of Wistar rats contain at least three organ-specific antigens as revealed by the gel diffusion analysis. Antiserum to rat testis absorbed with serum and liver cross-reacts with the extracts of epididymis, brain and ovary. Heating at over 60° C destroys the combining power of all the three testis-specific antigens. Two of the three organ-specific antigens of the Wistar strain of rats are common to two antigens present in the testes of man, sheep, rabbit, hamster, guinea-pig and mouse.

Free access

S. B. MOODBIDRI, A. R. SHETH and SHANTA S. RAO

Summary.

Binding of human placental [125I]lactogen to homogenates of the buffalo CL was studied. Differential centrifugation studies revealed the existence of hormone-binding components in fractions of the CL homogenate sedimenting at 800 g, 10,000 g and 100,000 g. The binding was affected by incubation time and hormone concentration. The hormone-binding components were heat-labile and susceptible to proteolytic enzymes.

Free access

V. K. Naik, U. M. Joshi and A. R. Sheth

Summary. The seminal plasma constituents of acid phosphatase, maltase, citric acid, prolactin, zinc and magnesium were measured in men vasectomized for 1–8 years. Compared with values obtained for a group of normal fertile men, all the constituents, except acid phosphatase which was unchanged, decreased significantly after vasectomy, but not progressively so. Since the constituents measured are believed to be of prostatic origin, the results suggest that vasectomy in men decreases the secretory function of the prostate gland.

Free access

A. N. THAKUR, A. R. SHETH, T. V. PURANDARE, S. R. MUNSHI and SHANTA S. RAO

Summary.

The effect of antisera to ovine LH and ovine prolactin was studied on polyamine levels in the mouse placenta. The antisera were administered on Day 11, 12 or 13 of pregnancy and the mice were killed 24 hr later. The polyamines (spermine, spermidine and putrescine) in the placentae were estimated. Polyamine levels were reduced after treatment with anti-LH on any of the 3 days and after treatment with anti-prolactin serum on Days 11 or 12. Only the spermidine content was reduced when anti-prolactin serum was injected on Day 13 of pregnancy. Placental DNA and RNA levels paralleled those observed for polyamine content. The changes in polyamine content and nucleic acid levels indicate that these antisera to LH and prolactin interfere with placental function.

Free access

Vijaya Raghavan, Tarala V. Purandare, A. R. Sheth and Safia R. Munshi

The circulating levels of gonadotrophins in immature and adult rats have been studied by several workers (Weisz & Ferin, 1970; Goldman, Grazia, Kamberi & Porter, 1971; Ojeda & Ramirez, 1972; Dohler & Wuttke, 1974, 1975). We have shown that administration of antigonadotrophic sera to neonatal mice affects ovarian development (Purandare, Munshi & Rao, 1976). In the present study the changes in gonadotrophin levels after treatment of neonatal mice wth such antisera were examined.

Free access

A. R. SHETH, G. V. SHAH, B. A. GADGIL and SHANTA S. RAO

Institute for Research in Reproduction (ICMR), Parel, Bombay-400 012, India

(Received 9th May 1974)

The bonnet monkey (Macaca radiata) of South India secretes large quantities of cervical mucus (Ovadia, McArthur, Kopito & Ulfelder, 1971) but little is known about its biochemical composition. Enzymes of cervical mucus have an important rôle to play by providing energy for sperm motility, survival and transport in the female genital tract.

In this study, an attempt was made to evaluate cyclical changes in the activities of glycogen phosphorylase, maltase and amylase, enzymes involved in the degradation of glycogen, and to compare these changes with alterations in serum LH levels during the menstrual cycle.

Adult female bonnet monkeys were housed individually in open wire cages. Upon arrival from dealers, the animals were examined, weighed (weights ranged between 2 and 4 kg), placed in quarantine, tested for tuberculosis and kept under close observation for a period of

Free access

Indira Chakravarty, Parul G. Shah, A. R. Sheth and J. J. Ghosh

Summary. Administration of delta-9-tetrahydrocannabinol (Δ9-THC) to prooestrous rats (5 mg/kg and 10 mg/kg, i.p. for 10 days) decreased the hypothalamic LH-RH content. Serum prolactin levels were reduced but serum LH and FSH and pituitary hormone content were similar to values in dioestrous rats. It is suggested that Δ9-THC acts primarily on the hypothalamus.