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A. T. GREGOIRE and R. ANSBACHER

Summary.

The presence of a unilateral IUD resulted in a uterotrophic effect in intact, pseudopregnant and ovariectomized hamsters with or without administration of 10 μg oestrogen. The glycogen content of the uterine horn containing the device was significantly less during pseudopregnancy. While the genital tract responded to oestrogen administration with glycogen synthesis, there was no enhancement of glycogen synthesis with the IUD. No difference in uterine water content was discovered in the presence of the IUD.

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A. T. GREGOIRE and A. E. RAKOFF

During recent investigations of the biochemical environment of the rabbit genital tract (Gregoire, Gongsakdi & Rakoff, 1961, 1962), the presence of urea was observed. It is generally stated that urea is synthesized in the liver exclusively and cannot be utilized by monogastric animals. Recently, however, evidence of extra-hepatic synthesis and utilization of urea has been published. Using radioactive nitrogen, Sporn, Dingman, Defalco & Davies (1959) demonstrated that urea may be synthesized by brain tissue. Rat hearts were found to metabolize urea and to form it when perfused with ammonium chloride (Menne, 1960), and synthesis of urea was observed in frog muscle (Nekhorocheff, 1952). Administration of urea labelled with 15N to rats resulted in nitrogen utilization for the synthesis of non-essential amino acids (Rose & Dekker, 1956).

Daily administration of progesterone to either

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A. T. GREGOIRE, H. RAMSEY and A. ADAMS

Summary.

Administration of oestradiol benzoate, 5 to 200 μg, intramuscularly daily for 3 days, results in glycogen deposition in the uterus and cervix of castrated Wistar rats. The relation between dose of oestrogen and amount of glycogen deposited is not, however, linear nor did all portions of the tract respond in a similar manner. The glycogen level of the vagina was not affected by the hormone administration, while progesterone lowered the glycogen content of all portions of the tract.

The glycogen metabolism of the mammalian female genital tract is briefly reviewed and discussed.

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A. T. GREGOIRE and B. J. GUINNESS

Summary.

The daily glycogen content of the uterus, cervix, upper and lower vagina and abdominal muscle of the hamster during the 4-day cycle was determined quantitatively by the anthrone method and localized histochemically with the PAS reaction. The amount of glycogen was maximal in the cervical and upper vaginal tissue on Day 1, the day preceding ovulation. While uterine and vaginal glycogen also increased significantly on Day 1, the increases were not as great as in the other portions of the tract.

A second group of hamsters was killed at 12.00, 16.00 and 20.00 hours on Day 4, 24.00, 12.00, 16.00 and 20.00 hours on Day 1, as well as at 24.00 hours on Day 2. Maximal glycogen levels were demonstrated 9 to 17 hr preceding ovulation at 08.00, 12.00 and 16.00 hours in the cervix and upper vagina. Only slight differences were observed in the uterus and lower vaginal tissue.

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A. T. GREGOIRE, D. H. DRISCOLL and A. ADAMS

Various attempts have been made to utilize the absorptive properties of the vagina for administration of therapeutic drugs. The vaginal absorption of organic elements, their subsequent isolation in urine, serum or manifestation of their endocrine effects, has been demonstrated with phenylmercuric acetate in the human (Eastman & Scott, 1944) and rat (Laug & Kunze, 1949), and with insulin in the dog (Fischer, 1923) but not the human (Woodyatt, 1922). Penicillin is absorbed within 60 min by the human vagina (Lovelady, Randall & Hosfeld, 1946; Goldberger, Walter & Lapid, 1947), while vaginal absorption of sulphanilamides exhibits daily variation (Carrington, Rohrer, Jones & Moore, 1944).

While investigating oestrogen metabolism, the uptake of tritiated oestrogens by spayed mouse genital tissues after intravenous (Stone, Baggett & Donnelly, 1963), subcutaneous (Stone, 1963) or

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R. H. FOOTE, H. D. HAFS, R. E. STAPLES, A. T. GREGOIRE and R. W. BRATTON

Summary.

At any one time a large proportion of individually caged, sexually mature female rabbits failed to copulate. Intravenous administration of purified pituitary luteinizing hormone (plh) (Armour) produced ovulations in sexually receptive and nonreceptive does alike. Does with histories of infertility ovulated after the injection of plh. The routine administration of 2·5 mg of plh to fifty-seven virgin and multiparous Dutch-Belted does averaging 2 to 3 kg, followed by artificial insemination, resulted in 91% of the does kindling and 307 young being born. Administration of either 0·5 mg or 1 mg of plh per kg to random members of fourteen pairs of does, accompanied by artificial insemination, resulted in no difference between the number of ovulations or the number of young per treatment (P>0·05). Fertility and litter size were normal when sixteen does were re-injected with plh and inseminated after weaning their previous litters.