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  • Author: ANNE STOCKELL HARTREE x
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ANNE STOCKELL HARTREE, JOHN B. MILLS, ROBERT A. S. WELCH and MARJORIE THOMAS

Summary.

A method developed for separation and partial purification of human pituitary hormones has been applied to acetone-dried horse pituitary glands. Growth hormone, follicle-stimulating hormone and luteinizing hormone activities were each obtained in separate fractions. Further purification of each hormone was achieved by the same or slightly modified procedures effective for the corresponding human hormones and the yields and potencies of the preparations were comparable to those obtained by other investigators. Hormones prepared from acetone-dried horse pituitaries stored for 25 years were of similar yield and potency to those prepared from recently collected glands. Horse and human luteinizing hormones cross-reacted immunologically and the horse hormone was assayed by an immunological method. Strong cross-reactions were observed between horse, pig and beef growth hormones and a very weak cross-reaction between horse and human growth hormones.

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C. H. J. Powell-Jones, J. B. Lester, C. Polge and Anne Stockell Hartree

Summary. Day 13–16 pig conceptus tissue was cultured for 24 h in medium containing [3H]leucine. The patterns of radioactively labelled proteins that were released into the medium during culture were analysed by SDS—polyacrylamide gel electrophoresis and fluorography. Day-13 conceptuses released two major radiolabelled proteins of Mr 23 000 and 26 000 and Day 14–16 conceptuses released these as well as proteins of Mr 14000, 19 000, 44000, 50 000 and 88 000. Various immunological and biological tests for a human chorionic gonadotrophin-like activity were performed on tissue extracts and on culture medium, but there was no evidence for its presence in the pig conceptus at Day 13–16 of gestation.