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The control of the formation of rabbit oviduct fluid and its relationship to the secretion of chloride ions has been studied using an in vitro vascularly perfused preparation. Fluid was produced at a rate of 43.41 μl h−1 in oviducts from rabbits in oestrus. The rate was increased by isoprenaline and tetraethylammonium, decreased by dibutyryl cAMP, dihydro4,4′diisothiocyanatostilbene-2,2′-disulfonic acid (H2DIDS), and propranolol, while amiloride had no effect. H2DIDS induced a small decrease and isoprenaline a small increase in vascular to lumen Cl− flux but propranolol and dibutyryl cAMP had no effect. Oviducts from pseudopregnant animals treated with hCG three days before the experiment produced significantly less fluid than did those from rabbits in oestrus, but there was no difference in vascular to lumen Cl− flux. The concentration of K+ in oviduct fluid formed in vitro was more than three times higher than in the vascular perfusate. The ability of adrenergic agents to influence the formation of rabbit oviduct fluid could have clinical implications in the prevention or treatment of female infertility due to blockage of the Fallopian tubes and might also be useful in enhancing the secretory activity of oviduct cells maintained in co-culture with early embryos.
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A pure population of rabbit oviduct epithelial cells was isolated and grown as a polarized monolayer on collagen-impregnated filters in primary culture. The cells were shown to be epithelial by immunocytochemical staining. The cells were mounted in a modified Ussing chamber which enabled ion transport across the cells to be studied. There was a net flux of Cl− in a basal to apical direction which was reversed by 1 mmol dibutyryl cyclic AMP l−1 (cAMP). A small but consistent transepithelial electrical potential difference (p.d.) of 0.86 mV was recorded with the apical side of the cells negative with respect to the basal. Adrenaline added to the basal side of the cells induced large transient increases in p.d. across the monolayer, involving both alpha and beta receptors. Adrenaline also induced a small increase in basal to apical Cl− transport across the cells. It is proposed that adrenergic agonists and cAMP modulate rabbit oviduct fluid formation in part via an effect on transepithelial chloride transport.
