Search Results

You are looking at 1 - 2 of 2 items for

  • Author: C. J. EPSTEIN x
Clear All Modify Search
Free access

C. J. EPSTEIN, C. W. SMITH and LILLIAN W. KWOK

Previous studies have indicated that the glucose-6-phosphate dehydrogenase (G6PD) contained within mouse ova is synthesized before ovulation and is under the genetic control of the X-chromosome (Epstein, Wegienka & Smith, 1969; Epstein, 1969). As such, it is different from the autosomally controlled hexose-6-phosphate dehydrogenase (H6PD) or glucose dehydrogenase found in liver microsomes but not in erythrocytes (Beutler & Morrison, 1967). To establish the applicability of these genetic results with ova to other tissues of the mouse, G6PD from ova has been characterized and compared with erythrocyte G6PD. Ova and pre-implantation embryos were obtained from Swiss albino mice (Mus musculus) by superovulation. For microspectrophotometric assay of enzyme activity, they were lysed by freezing and thawing in 0·05 m-tris buffer, pH 7·5, containing 0·05% bovine serum albumin (BSA) (Epstein et al., 1969). For polyacrylamide disc
Free access

H. SPIELMANN, R. P. ERICKSON and C. J. EPSTEIN

Summary.

Lactate dehydrogenase and glucose-6-phosphate dehydrogenase in Day-1 and Day-4 preimplantation mouse embryos was assayed by immunotitration. For both enzymes, immunoreactive protein was found to decrease in parallel with enzyme activity, indicating that loss of activity is probably the result of enzyme degradation. The identification of embryonic lactate dehydrogenase as isoenzyme LDH-1 and of glucose-6-phosphate dehydrogenase as the form found in red cells is corroborated by the immunological data.