Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Carolina Marvaldi x
  • Refine by access: All content x
Clear All Modify Search
Carolina Marvaldi Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Carolina Marvaldi in
Google Scholar
PubMed
Close
,
Felisa Herrero Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Felisa Herrero in
Google Scholar
PubMed
Close
,
Clare Johnson Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, USA

Search for other papers by Clare Johnson in
Google Scholar
PubMed
Close
,
Julieta Aylen Schander Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Julieta Aylen Schander in
Google Scholar
PubMed
Close
,
Fernando Correa Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Fernando Correa in
Google Scholar
PubMed
Close
,
Maximiliano Cella Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Maximiliano Cella in
Google Scholar
PubMed
Close
,
Julieta Aisemberg Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Julieta Aisemberg in
Google Scholar
PubMed
Close
,
Ana María Franchi Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Ana María Franchi in
Google Scholar
PubMed
Close
,
Heather Bradshaw Department of Psychological and Brain Sciences, Indiana University, Bloomington, Indiana, USA

Search for other papers by Heather Bradshaw in
Google Scholar
PubMed
Close
, and
Manuel Luis Wolfson Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos, Universidad de Buenos Aires-Consejo Nacional de Investigaciones en Ciencia y Técnica, Ciudad Autónoma de Buenos Aires, Argentina

Search for other papers by Manuel Luis Wolfson in
Google Scholar
PubMed
Close

In brief

The cervix plays a crucial role not only in the maintenance of pregnancy but also during delivery, when it undergoes extensive changes. This study highlights the involvement of the endocannabinoidome in cervical remodeling, emphasizing its relevance in the shift from a nonpregnant to pregnant state and its potential contribution to preterm delivery in inflammatory contexts.

Abstract

During pregnancy, the main role of the cervix is to isolate the fetus from outside pathogens and maintain the relatively closed system of uterine gestation. Conversely, toward the end of pregnancy, the cervix must be remodeled to increase flexibility and allow the delivery. This process is called cervical remodeling and dysregulation of the process plays a role in premature delivery. The endocannabinoidome plays an important role in several reproductive events; however, its function on cervical tissue throughout pregnancy is poorly understood. The goal of this study was to evaluate the presence and participation of the endocannabinoidome in lipopolysaccharide (LPS)-induced cervical changes. Therefore, we evaluated key components of the endocannabinoidome in cervical tissue from nonpregnant mice and pregnant mice with and without LPS treatment. Using mass spectrometric analysis, we found an increase in anandamide and 2-arachidonoylglycerol in the cervix of pregnant mice when compared to nonpregnant mice. We have also found a reduction in FAAH protein expression in these tissues. Furthermore, when treated with LPS, we observed a reduction in the cervical immunostaining with anti-CB1 and anti-CB2 antibodies. Likewise, using cervix explants from pregnant mice, we found that LPS significantly increased cervical metalloprotease activity and cyclooxygenase 2, which were subsequently modulated by cannabinoid receptor antagonists. Collectively, our findings suggest that an LPS-induced imbalance of cervix endocannabinoidome likely contributes to premature cervical remodeling, which is part of the key components that contribute to premature delivery.

Restricted access
Carolina Marvaldi Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Fisiopatología de la Preñez y el Parto, Centro de Estudios Farmacológicos y Botánicos (CEFyBO) UBA-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Carolina Marvaldi in
Google Scholar
PubMed
Close
,
Daniel Martin Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biología Celular e Histología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Daniel Martin in
Google Scholar
PubMed
Close
,
Julia G Conte Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Julia G Conte in
Google Scholar
PubMed
Close
,
María Florencia Gottardo Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Oncología Molecular, Universidad Nacional de Quilmes, Buenos Aires, Argentina

Search for other papers by María Florencia Gottardo in
Google Scholar
PubMed
Close
,
Matías L Pidre Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata – CONICET, La Plata, Argentina

Search for other papers by Matías L Pidre in
Google Scholar
PubMed
Close
,
Mercedes Imsen Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Mercedes Imsen in
Google Scholar
PubMed
Close
,
Martin Irizarri Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biología Celular e Histología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Martin Irizarri in
Google Scholar
PubMed
Close
,
Sharron L Manuel Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA

Search for other papers by Sharron L Manuel in
Google Scholar
PubMed
Close
,
Francesca E Duncan Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA

Search for other papers by Francesca E Duncan in
Google Scholar
PubMed
Close
,
Víctor Romanowski Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata – CONICET, La Plata, Argentina

Search for other papers by Víctor Romanowski in
Google Scholar
PubMed
Close
,
Adriana Seilicovich Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biología Celular e Histología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Adriana Seilicovich in
Google Scholar
PubMed
Close
, and
Gabriela Jaita Instituto de Investigaciones Biomédicas (INBIOMED) UBA-CONICET, Facultad de Medicina, Uiversidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biología Celular e Histología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

Search for other papers by Gabriela Jaita in
Google Scholar
PubMed
Close

Humanin (HN) is a short peptide involved in many biological processes such as apoptosis, cell survival, inflammatory response, and reaction to stressors like oxidative stress, between others. In the ovary, a correct balance between pro- and anti-apoptotic factors is crucial for folliculogenesis. In the follicular atresia, survival or death of granulosa cells is a critical process. The goal of this study was to evaluate the action of HN on granulosa cell fate. To explore endogenous HN function in the ovary, we used a recombinant baculovirus (BV) encoding a short-hairpin RNA targeted to silence HN (shHN). HN downregulation modified ovarian histoarchitecture and increased apoptosis of granulosa cells. HN was also detected in a granulosa tumor cell line (KGN). Transduction of KGN cells with BV-shHN resulted in HN downregulation and increased apoptosis. On the other hand, treatment of KGN cells with exogenous HN increased cell viability and decreased apoptosis. In summary, these findings indicate that HN is a cytoprotective factor in granulosa cells of antral follicles, suggesting that this peptide would be involved in the regulation of folliculogenesis. Also, this peptide is a cytoprotective factor in KGN cells, and therefore, it could be involved in granulosa tumor cell behavior.

Restricted access