Search Results
You are looking at 1 - 10 of 30 items for
- Author: Chen Yang x
- Refine by access: All content x
Search for other papers by Chen Geng in
Google Scholar
PubMed
Search for other papers by Hao-ran Liu in
Google Scholar
PubMed
Search for other papers by Yue Zhao in
Google Scholar
PubMed
Search for other papers by Yang Yang in
Google Scholar
PubMed
Search for other papers by Lan Chao in
Google Scholar
PubMed
The epithelial-to-mesenchymal transition may play a role in adenomyosis. GRIM19 expression is downregulated in adenomyotic lesions, and the effects of this downregulation in adenomyosis remain relatively unclear. In this study, we aimed to explore whether aberrant GRIM19 expression is associated with the epithelial-to-mesenchymal transition in adenomyosis and found that the expression of both GRIM19 and WT1 was low, and epithelial-to-mesenchymal transition, which included significant changes in CDH1, CDH2 and KRT8 expression, occurred in adenomyotic lesions, as confirmed by Western blotting and quantitative real-time PCR. We provided novel insights into WT1 expression in adenomyosis, revealing that WT1 expression was increased in the endometrial glands of adenomyotic lesions by immunohistochemistry. In vitro, knockdown of GRIM19 expression by small interfering RNA (siRNA) promoted the proliferation, migration and invasion of Ishikawa cells, as measured by Cell Counting Kit-8, wound healing assay and Transwell assays. Western blotting and quantitative real-time PCR confirmed that WT1 expression increased and epithelial-to-mesenchymal transition was induced, including the upregulation of CDH2 and downregulation of CDH1 and KRT8after transfecting the GRIM19 siRNA to Ishikawa cells. Furthermore, Wt1 expression was upregulated and epithelial-to-mesenchymal transition was observed, including downregulation of Cdh1 and Krt8 in Grim19 gene-knockdown mice. Upregulation of Wt1 expression in the endometrial glands of Grim19 knockdown mice was also verified by immunohistochemistry. Taken together, these results reveal that low expression of GRIM19 in adenomyosis may upregulate WT1 expression and induce epithelial-to-mesenchymal transition in the endometria, providing new insights into the pathogenesis of adenomyosis.
Search for other papers by Jingmei Hou in
Google Scholar
PubMed
Search for other papers by Shi Yang in
Google Scholar
PubMed
Search for other papers by Hao Yang in
Google Scholar
PubMed
Search for other papers by Yang Liu in
Google Scholar
PubMed
Search for other papers by Yun Liu in
Google Scholar
PubMed
Search for other papers by Yanan Hai in
Google Scholar
PubMed
Search for other papers by Zheng Chen in
Google Scholar
PubMed
Search for other papers by Ying Guo in
Google Scholar
PubMed
Search for other papers by Yuehua Gong in
Google Scholar
PubMed
Search for other papers by Wei-Qiang Gao in
Google Scholar
PubMed
Search for other papers by Zheng Li in
Google Scholar
PubMed
State Key Laboratory of Oncogenes and Related Genes, Department of Urology, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai Key Laboratory of Reproductive Medicine, Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, China
State Key Laboratory of Oncogenes and Related Genes, Department of Urology, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai Key Laboratory of Reproductive Medicine, Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, China
State Key Laboratory of Oncogenes and Related Genes, Department of Urology, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai Key Laboratory of Reproductive Medicine, Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, China
Search for other papers by Zuping He in
Google Scholar
PubMed
Infertility is a major and largely incurable disease caused by disruption and loss of germ cells. It affects 10–15% of couples, and male factor accounts for half of the cases. To obtain human male germ cells ‘especially functional spermatids’ is essential for treating male infertility. Currently, much progress has been made on generating male germ cells, including spermatogonia, spermatocytes, and spermatids, from various types of stem cells. These germ cells can also be used in investigation of the pathology of male infertility. In this review, we focused on advances on obtaining male differentiated germ cells from different kinds of stem cells, with an emphasis on the embryonic stem (ES) cells, the induced pluripotent stem (iPS) cells, and spermatogonial stem cells (SSCs). We illustrated the generation of male differentiated germ cells from ES cells, iPS cells and SSCs, and we summarized the phenotype for these stem cells, spermatocytes and spermatids. Moreover, we address the differentiation potentials of ES cells, iPS cells and SSCs. We also highlight the advantages, disadvantages and concerns on derivation of the differentiated male germ cells from several types of stem cells. The ability of generating mature and functional male gametes from stem cells could enable us to understand the precise etiology of male infertility and offer an invaluable source of autologous male gametes for treating male infertility of azoospermia patients.
Search for other papers by Jing Liu in
Google Scholar
PubMed
Search for other papers by Yang Wang in
Google Scholar
PubMed
Search for other papers by Peng Chen in
Google Scholar
PubMed
Search for other papers by Yue Ma in
Google Scholar
PubMed
Search for other papers by Shuo Wang in
Google Scholar
PubMed
Search for other papers by Ye Tian in
Google Scholar
PubMed
Search for other papers by Anna Wang in
Google Scholar
PubMed
Search for other papers by Danbo Wang in
Google Scholar
PubMed
Previous lncRNA microarray screening found that the AC002454.1 gene was highly expressed in endometriosis (EMS), and these expression levels were highly correlated with cyclin-dependent kinase-6 (CDK6). This study investigated the expression level and correlation between AC002454.1 and CDK6 in endometrium tissues and the influence of these changes in expression upon the biological behavior of eutopic endometrial cells. We confirmed AC002454.1 and CDK6 mRNA and protein were highly expressed in ectopic and eutopic endometrial tissue from patients with EMS and were clearly correlated. In vitro, both AC002454.1 and CDK6 positively regulated the proliferation, migration and invasion ability of eutopic endometrial cells and could promote the transformation of cells from G0/G1 phase to S phase. AC002454.1 and CDK6 may have synergistic effects, thereby affecting the biological behavior of endometrial cells, and thus promote the progression of EMS.
Search for other papers by Chen Yang in
Google Scholar
PubMed
Search for other papers by Yue Li in
Google Scholar
PubMed
Search for other papers by Hai-Yang Pan in
Google Scholar
PubMed
Search for other papers by Meng-Yuan Li in
Google Scholar
PubMed
Search for other papers by Ji-Min Pan in
Google Scholar
PubMed
Search for other papers by Si-Ting Chen in
Google Scholar
PubMed
Search for other papers by Hai-Yi Zhang in
Google Scholar
PubMed
Search for other papers by Zhen-Shan Yang in
Google Scholar
PubMed
Search for other papers by Hai-Ting Dou in
Google Scholar
PubMed
Search for other papers by Zeng-Ming Yang in
Google Scholar
PubMed
There are around 300 million adolescent pregnancies worldwide, accounting for 11% of all births worldwide. Accumulating evidence demonstrates that many adverse perinatal outcomes are associated with adolescent pregnancies. However, how and why these abnormalities occur remain to be defined. In this study, pregnancy at different stages was compared between 25- and 30- day-old and mature female mice. We found that the litter size of adolescent pregnancy is significantly decreased from F1 to F3 generations compared to mature pregnancy. On days 8 and 12 of pregnancy, multiple abnormalities in decidual and placental development appear in F3 adolescent pregnancy. On days 5 and 8, uterine endoplasmic reticulum stress is dysregulated in F3 adolescent pregnancy. Embryo implantation and decidualization are also compromised in adolescent pregnancy. Many genes are abnormally expressed in adolescent estrous uteri. The abnormal endocrine environment and abnormal implantation from uterine immaturity may result in multiple pregnancy failures in adolescent pregnancy. The aim of this study is to shed light on human adolescent pregnancy.
Search for other papers by Zi-gang Shen in
Google Scholar
PubMed
Search for other papers by Wei He in
Google Scholar
PubMed
Search for other papers by Ji Zhang in
Google Scholar
PubMed
Search for other papers by Hai-yang He in
Google Scholar
PubMed
Search for other papers by Xia Yang in
Google Scholar
PubMed
Search for other papers by Zheng-qiong Chen in
Google Scholar
PubMed
Search for other papers by Ping Yang in
Google Scholar
PubMed
Search for other papers by Jian Li in
Google Scholar
PubMed
Search for other papers by Zhi-qing Liang in
Google Scholar
PubMed
Search for other papers by Yu-zhang Wu in
Google Scholar
PubMed
Search for other papers by Jin-tao Li in
Google Scholar
PubMed
SPINLW1 (previously known as eppin (epididymal protease inhibitor)) is a target under intense scrutiny in the study of male contraceptive vaccines. B-cell-dominant epitopes are now recognized as key parts of the induction of humoral immune responses against target antigens. The generation of robust humoral responses in vivo has become a crucial problem in the development of modern vaccines. In this study, we developed a completely novel B-cell-dominant-epitope-based mimovirus vaccine, which is a kind of virus-size particulate antigen delivery system. The mimovirus successfully self-assembled from a cationic peptide containing a cell-penetrating peptide of TAT49–57 and a plasmid DNA encoding both three SPINLW1 (103–115) copies and adjuvant C3d3. The male mice were immunized with the epitope-based mimovirus vaccine, which resulted in a gradual elevation of specific serum IgG antibody levels. These reached a peak at week 4. Mating for the fertility assay showed that the mimovirus vaccine had accomplished a moderate fertility inhibition effect and investigation into the mechanism of action showed that it did so by interfering with the reproductive function of the sperm but that it did not damage the structures of the testes or cause serum testosterone to decline. Our results suggest an ideal protocol for suppressing fertility in mice by an engineered mimovirus vaccine.
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Feiyan Zhao in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Tong Chen in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Xuehan Zhao in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Qin Wang in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Yonglian Lan in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Yu Liang in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Ying Li in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Shuyu Wang in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Yang Yang in
Google Scholar
PubMed
Department of Human Reproductive Medicine, Beijing Maternal and Child Health Care Hospital, Beijing, China
Search for other papers by Xiaokui Yang in
Google Scholar
PubMed
Recurrent implantation failure (RIF) is a challenge in the field of reproductive medicine, but mechanisms for its occurrence remain still unclear. Long non-coding RNAs (lncRNAs) have been found to play a vital role in many different diseases. In recent years, the differentially expressed lncRNAs have been reported in endometrial tissues. Here, we profiled dysregulated lncRNAs and mRNAs in the endometrial tissues of RIF patients and performed correlation analysis. We found that LINC02190 was upregulated in RIF endometrium and was bound to the integrin αD (ITGAD) mRNA promoter. Immunofluorescence assays were used to detect the location of ITGAD in the Ishikawa cell line and patients’ endometrial biopsies. Overexpressed LINC02190 could decrease the expression of ITGAD and the adhesion rate of Ishikawa and JAR cells. Knockdown of the expression of LINC02190 significantly increased the ITGAD level, as well as the adhesion rate of Ishikawa and JAR cells. Furthermore, we demonstrated that the 150–250 bps of LINC02190 were the cis-elements involved in the regulation of ITGAD promoter activities. In conclusion, the results demonstrated that LINC02190 plays an important role in the occurrence of RIF, and the molecular mechanism may be associated with the embryo–endometrial attachment mediated by ITGAD. This study emphasizes the importance of lncRNAs in the occurrence of RIF and provides a potential new biomarker for diagnosis and therapies.
Search for other papers by Z-M. Yang in
Google Scholar
PubMed
Search for other papers by S-P. Le in
Google Scholar
PubMed
Search for other papers by D-B. Chen in
Google Scholar
PubMed
Search for other papers by K. Yasukawa in
Google Scholar
PubMed
Search for other papers by M. J. K. Harper in
Google Scholar
PubMed
The presence of leukaemia inhibitory factor (LIF) binding and expression of the gp130 component of the LIF receptor were studied in the rabbit uterus during early pregnancy. LIF binding to myometrium was moderate in oestrous and non-oestrous animals and on day 1 of pregnancy, declined on days 2 and 3, and increased to a peak value on days 5 and 6 of pregnancy. Binding to stromal cells was not observed. Binding of LIF to luminal and glandular epithelium was low in unmated animals and on days 1 and 2 of pregnancy. Binding to luminal epithelium increased from day 3, and to glandular epithelium from day 5 of pregnancy. Highest binding was seen on days 5 and 6, with a slight decline observed on day 7, and with little difference between the mesometrial and antimesometrial regions of the implantation site. In all cases, binding of LIF was similar in the uteri of day 6 pseudopregnant and pregnant animals. At all stages, gpl30 was absent from stroma and almost absent from myometrium and glandular epithelium. It was expressed in luminal epithelium, reaching maximal expression on day 6 of pregnancy and pseudopregnancy, but diminished on day 7 of pregnancy, particularly in the antimesometrial area of the implantation site. The coexpression of LIF binding and gpl30 may indicate the presence of high-affinity LIF receptor, which matches the pattern of LIF protein expression and, as in mice, suggests its importance for implantation.
Shaanxi Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Northwest A&F University, Yangling, China
Search for other papers by Daqian Dong in
Google Scholar
PubMed
Shaanxi Stem Cell Engineering Research Center, Northwest A&F University, Yangling, China
Search for other papers by Jinmeng Yang in
Google Scholar
PubMed
Search for other papers by Yining Chen in
Google Scholar
PubMed
Search for other papers by Guofan Peng in
Google Scholar
PubMed
Shaanxi Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Northwest A&F University, Yangling, China
Search for other papers by Heran Cao in
Google Scholar
PubMed
Shaanxi Stem Cell Engineering Research Center, Northwest A&F University, Yangling, China
Search for other papers by Huihui Gao in
Google Scholar
PubMed
Search for other papers by Tianqi Jin in
Google Scholar
PubMed
Shaanxi Stem Cell Engineering Research Center, Northwest A&F University, Yangling, China
Search for other papers by Fangxia Yang in
Google Scholar
PubMed
Shaanxi Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Northwest A&F University, Yangling, China
Search for other papers by Wuzi Dong in
Google Scholar
PubMed
Epididymal specific proteins play a crucial role in sperm maturation. Some of the post-translational modified proteins are transported from the caput to the cauda of the epididymis through exosomes which regulate the function of sperm in cauda epididymis. Rat beta-galactosidase-1-like protein 4 (GLB1L4) expressed specifically in the caput epididymis, localizes on the sperm; however, the regulatory ways in which GLB1L4 protein interacts with sperm to maintain sperm function are unclear. In this study, knockdown of rat GLB1L4 could inhibit in vitro capacitation of sperm in cauda epididymis and reduce the fertility of the male rats by injection of special lentivirus-shRNA into caput epididymis. Moreover, a considerable proportion of GLB1L4 proteins from rat caput epididymis were loaded on exosomes. The exosomes loaded GLB1L4 from in vitro primary rat caput epididymal epithelial cells could bind with spermatozoa in cauda epididymis. Further, the palmitoylation status of cysteine residues at the 12th and 15th sites of the protein molecule could significantly affect cellular localization of GLB1L4 protein. It was identified that most of GLB1L4 was palmitoylated in the presence of exosomes from primary caput epididymal cells and the level of palmitoylated GLB1L4 in the exosomes could be inhibited by 2-bromopalmitate (2-BP). These results suggested that the palmitoylated GLB1L4 from rat caput epididymis could be transported to the cauda epididymis to regulate the sperm function by exosomes.
Search for other papers by Hu Gao in
Google Scholar
PubMed
Search for other papers by Bin Chen in
Google Scholar
PubMed
Search for other papers by Hui Luo in
Google Scholar
PubMed
Search for other papers by Bo Weng in
Google Scholar
PubMed
Search for other papers by Xiangwei Tang in
Google Scholar
PubMed
Search for other papers by Yao Chen in
Google Scholar
PubMed
Search for other papers by Anqi Yang in
Google Scholar
PubMed
Search for other papers by Maoliang Ran in
Google Scholar
PubMed
Sertoli cells are indispensable for normal spermatogenesis, and increasing evidence has shown that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and regulatory mechanisms of miRNAs in Sertoli cells of domestic animals have not been fully investigated. In the present study, we mainly investigated the regulatory roles of miR-499 in immature porcine Sertoli cells. The results showed that miR-499 was mainly located in the basement section of seminiferous tubules of prepubertal porcine testicular tissue. Overexpression of miR-499 promoted cell proliferation and inhibited apoptosis, whereas miR-499 inhibition resulted in the opposite effect. The PTEN gene was directly targeted by miR-499, and the expression of mRNA and protein was also negatively regulated by miR-499 in immature porcine Sertoli cells. siRNA-induced PTEN knockdown resulted in a similar effect as an overexpression of miR-499 and abolished the effects of miR-499 inhibition on immature porcine Sertoli cells. Moreover, both miR-499 overexpression and the PTEN knockdown activated the PI3K/AKT signaling pathway, whereas inhibition of the PI3K/AKT signaling pathway caused immature porcine Sertoli cell apoptosis and inhibited cell proliferation. Overall, miR-499 promotes proliferation and inhibits apoptosis in immature porcine Sertoli cells through the PI3K/AKT pathway by targeting the PTEN gene. This study provides novel insights into the effects of miR-499 in spermatogenesis through the regulation of immature Sertoli cell proliferation and apoptosis.
Search for other papers by Wenjun Wang in
Google Scholar
PubMed
Search for other papers by Hong Chen in
Google Scholar
PubMed
Search for other papers by Ruiqi Li in
Google Scholar
PubMed
Search for other papers by Nengyong Ouyang in
Google Scholar
PubMed
Search for other papers by Jinghua Chen in
Google Scholar
PubMed
Search for other papers by Lili Huang in
Google Scholar
PubMed
Search for other papers by Meiqi Mai in
Google Scholar
PubMed
Search for other papers by Ningfeng Zhang in
Google Scholar
PubMed
Search for other papers by Qingxue Zhang in
Google Scholar
PubMed
Search for other papers by Dongzi Yang in
Google Scholar
PubMed
Our previous study has demonstrated that luteinized granulosa cells (GCs) have the potential to proliferate and that the telomerase activity (TA) of luteinized GCs may predict the clinical outcomes of IVF treatment. However, in the field of telomere research, there have always been different opinions regarding the significance of TA and telomere length (TL). Thus, in the present study, we compared the effects of these two parameters on IVF treatment outcomes in the same individuals. TL did not differ significantly between the pregnant group and the non-pregnant group. The TA, number of retrieved oocytes and rate of blastocyst transfer were significantly higher in the pregnant group than in the non-pregnant group (0.8825 OD×mm, 12.75±2.20 and 34.48%, respectively, in the pregnant group vs 0.513 OD×mm, 11.60±0.93 and 14.89%, respectively, in the non-pregnant group (P<0.05)), while basal FSH level was lower in the pregnant group than in the non-pregnant group. The subjects did not differ with regard to ovarian stimulation or other clinical characteristics. A TA increase of 1 OD×mm increased the chance of becoming pregnant 4.769-fold (odds ratio: 5.769, 95% CI: 1.434–23.212, P<0.014). The areas under the receiver operating characteristic curves were 0.576 for TL and 0.674 for TA (P=0.271 and P<0. 012 respectively). The corresponding cut-off points were 4.470 for TL and 0.650 OD×mm for TA. These results demonstrate that TA is a better predictor of pregnancy outcomes following IVF treatment than TL. No other clinical parameters, including age, baseline FSH level or peak oestradiol level, distinguished between the pregnant group and the non-pregnant group as effectively as TA.
