It is the purpose of this study to determine the effect of relative centrifugal force (RCF) × g on various semen characteristics of the bull and boar.
Entire ejaculates of mature Yorkshire boars were collected and separated from the gel fraction by allowing the semen to run through three layers of cheesecloth into a warm thermos. The ejaculate was then placed in 50-ml centrifuge tubes and centrifuged at 1000g for 10 or 15 min to remove the spermatozoa. Part of the first supernatant was kept for further analyses, while the remaining semen was centrifuged at forces and times given in Tables 1, 2 and 3. Bull epididymal fluid was collected by flushing through the vas deferens with a 154 mm-KCl, 10 mm-tris buffer at pH 7·2 according