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  • Author: D. P. SADHU x
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BHUPAL SINGH, B. B. MAHAPATRO and D. P. SADHU

The present study was undertaken in view of the scanty information available concerning the chemistry of buffalo semen, the low survival of buffalo spermatozoa in storage compared with other cattle (Roy, Pandey & Rawat, 1960; Singh, 1967) and a report of a greater influence of adverse climatic conditions on the quality of buffalo semen compared with other cattle (Sinha, Gupta & Roy, 1966). Semen was collected from four Murrah buffalo and four Hariana bulls and the study was made during the seasons mentioned in Table 1. Immediately after collection, semen was stored in a thermos flask containing ice. The spermatozoa and seminal plasma were separated by centrifuging a known volume of pooled semen at low temperature for half an hour. The chemical constituents were determined according to the standard methods described by Hawk, Oser & Summerson (1954). The sodium content of the seminal plasma of the two species