E. B. BELL
E. B. BELL and ANNE McLAREN
Mouse spermatozoa were disrupted in a pressure cell and separated by centrifugation at 1200 g into supernatant and sediment components. Sperm head nuclei were isolated from the sediment by a sucrose gradient ultracentrifugation procedure. Injection of the alumprecipitated supernatant fraction into female mice significantly reduced both litter size (P<0·001) and fertilization rate (P<0·001). Fertility was depressed to a lesser degree by the same antigen when it had not been precipitated. The injection of isolated sperm-head nuclei had no significant effect on litter size or fertilization rate.
Serum antibody against spermatozoa was measured by passive haemagglutination as well as sperm agglutination. Passive haemagglutination titres were significantly higher in mice injected with supernatant preparations than in those receiving whole spermatozoa or sediment fractions. Freezing the supernatant before injection abolished the sperm agglutination titre but did not reduce the degree of impairment of fertility, indicating that sperm agglutinins were not responsible for the induced infertility.
G. L. FOSS, E. T. BELL, F. J. W. LEWIS, J. A. LORAINE and B. R. POLLARD
The effect of clomiphene on the sperm count and hormone excretion is described in a patient with Klinefelter's syndrome, having a 47 XXY karyotype, and in whom a testicular biopsy showed small areas of spermatogenesis.
Clomiphene stimulated the production of morphologically normal motile spermatozoa in the absence of any marked effect on hormone output.
Possible explanations for these findings are discussed.