E. SCHILLING and W. HOLM
W. JÖCHLE and E. SCHILLING
E. SCHILLING and J. ZUST
During the oestrus of the sow it has been demonstrated that the pH intra vaginam decreases rapidly into the acid range (Schilling & Röstel, 1964). The minimal pH of 6·35 coincided with full oestrus and the time of mating. These findings have now been obtained in cows (Zust, 1966; Schilling & Zust, 1967). The most important results of these investigations are briefly dealt with in the following text.
In cows, the pH was measured intra vaginam using a pocket-type pH meter. An extra long electrode was moistened with distilled water immediately before the measurement was made. A Polanski speculum was used to locate exactly the measurement points within the vagina. In accordance with Täubrich (1959) a close relationship was found between these points and the pH: the lowest values
A. A. El-Banna, B. Sacher and E. Schilling
Treatment of rabbits with indomethacin (10 mg/kg/day) 48 hr before mating, and with 20 mg/kg at 12 hr followed by 8 mg/kg at 48, 72 or 96 hr after mating did not affect the rate of egg transport through the oviduct. Indomethacin treatment at the time of implantation interfered with pregnancy and caused degeneration and resorption of embryos. These results suggest that inhibition of prostaglandin synthesis does not directly affect egg transport, but that prostaglandin appears to be required for the retention of implanted embryos.
E. Hinsch, W. Hägele, R-M. Bohle, W-B. Schill and K-D. Hinsch
The mouse zona pellucida protein ZP2 plays an important role in the process of fertilization by mediating secondary sperm binding to mammalian oocytes. ZP2 primary structures are highly conserved as revealed by cDNA cloning. The aim of the study was to identify ZP2 domains of functional relevance. Antisera were raised against synthetic peptides that are either conserved in the structure of ZP2 from different mammalian species (AS ZP2–20) or present in the human ZP2 but not in the mouse ZP2 amino acid sequence (AS ZP2–26). Antibody binding to zona pellucida proteins was assessed by assaying the antisera with human hemizonae. Using human zonae pellucidae, we demonstrated that anti-ZP2 common antibodies and anti-ZP2 human peptide antibodies react with human zona pellucida antigens. For the first time, ZP2 domains of functional relevance for human sperm–oocyte interaction could be identified applying the competitive hemizona assay. Antiserum AS ZP2–20 significantly inhibited binding of spermatozoa to test hemizonae, whereas treatment of hemizonae with AS ZP2–26 did not influence sperm–oocyte interaction. These results show that antibodies against synthetic ZP2 peptides react with ZP2 protein and that AS ZP2–20 identifies a linear ZP2 epitope that is of possible functional importance for sperm–oocyte interaction.