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EUGENIA ALUMOT and H. SCHINDLER

In a study of the composition of the epididymal fluid of rams, attention was given to the fact that nitrogen compounds constitute a great part of the dry matter and that so far seventeen free amino acids have been detected in the fluid (Ch. Neumark, unpublished data). It was therefore deemed of interest to study the enzymes of their metabolism. Two principal transaminases were investigated: glutamic-aspartic transaminase (got) and glutamic-alanine transaminase (gpt). The presence of these enzymes has been established in human, bovine and rabbit seminal plasma (Flipse, 1960; Povoa & Villela, 1960; Gregoire, Rakoff & Ward, 1961), but no report on their presence in the epididymal fluid was found in the literature.

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Epididymal fluid obtained from fistulated rams (A. Tadmor & H. Schindler, unpublished information) served for the enzyme determination. For comparison, the enzymatic activity was also investigated in the seminal plasma and blood serum of rams.

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EUGENIA ALUMOT, Y. LENSKY and H. SCHINDLER

Summary.

Proteins of ram epididymal fluid, seminal plasma, accessory gland fluid and blood serum were fractionated by electrophoresis. Nineteen bands of different mobilities were observed by disc electrophoresis. Immunoelectrophoresis revealed sixteen precipitation arcs in epididymal fluid and seminal plasma and fourteen in blood serum, following absorption with the antiserum against epididymal fluid. These results indicate that most of the epididymal fluid and seminal plasma proteins of the ram have antigens in common with blood serum.