In a study of the composition of the epididymal fluid of rams, attention was given to the fact that nitrogen compounds constitute a great part of the dry matter and that so far seventeen free amino acids have been detected in the fluid (Ch. Neumark, unpublished data). It was therefore deemed of interest to study the enzymes of their metabolism. Two principal transaminases were investigated: glutamic-aspartic transaminase (got) and glutamic-alanine transaminase (gpt). The presence of these enzymes has been established in human, bovine and rabbit seminal plasma (Flipse, 1960; Povoa & Villela, 1960; Gregoire, Rakoff & Ward, 1961), but no report on their presence in the epididymal fluid was found in the literature.
Epididymal fluid obtained from fistulated rams (A. Tadmor & H. Schindler, unpublished information) served for the enzyme determination. For comparison, the enzymatic activity was also investigated in the seminal plasma and blood serum of rams.