Search Results

You are looking at 1 - 2 of 2 items for

  • Author: F. R. BLATCHLEY x
  • Refine by Access: All content x
Clear All Modify Search
Free access


The changes in luteal function during pregnancy and pseudopregnancy in the ferret have previously been assessed by the study of the morphology of the ovaries and uterine tract (Hammond & Marshall, 1930; Donovan, 1967) but, with the development of competitive protein-binding techniques, the measurement of progesterone in small quantities of blood has become possible. We have used this procedure to assess progestin secretion by ovarian tissue in anoestrous, oestrous and pseudopregnant ferrets, and also after hypophysectomy.

Blood samples (2 ml), withdrawn by cardiac puncture under Nembutal anaesthesia, were centrifuged as soon as possible after collection and the plasma was stored at −20° C until assay. Plasma progestin concentrations were measured in 0·1-ml aliquots in triplicate as described previously (Blatchley, Donovan, Horton & Poyser, 1972), the limit of sensitivity being 0·8

Free access


Evidence is accumulating (Blatchley & Donovan, 1973) that the uterine luteolytic hormone in mammals is prostaglandin F (PGF). This prostaglandin is released from the guinea-pig uterus under conditions associated with premature luteolysis, such as bead insertion (Poyser, Horton, Thompson & Los, 1971) and oestrogen treatment (Blatchley, Donovan, Poyser, Horton, Thompson & Los, 1971), and both the synthesis and release of PGF increase toward the end of the oestrous cycle when natural luteal regression occurs (Poyser, 1972; Blatchley, Donovan, Horton & Poyser, 1972). The factors involved in the regulation of the cyclic release of prostaglandin from the uterus have not been extensively studied, although Pickles (1967) suggested that progesterone may regulate the synthesis of prostaglandins in the human uterus as the concentration