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  • Author: G. PINCUS x
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Hamner & Fox (1969) have recently reviewed various techniques for the collection of oviduct fluid in the rabbit, sheep, pig and monkey. Continual collection of the oviduct fluid from the rhesus monkey was first achieved by Mastroianni, Shah & Abdul-Karim (1961). They cannulated the oviducts with silicone rubber tubings which were connected to an external volumetric collection device fixed to the skin. Movements of the animals' upper limbs were restricted with plaster to prevent the animals from reaching the device. Using this technique, daily samples were collected for as long as four complete menstrual cycles.

In view of the possibility that a physical restriction of the monkey might cause a deleterious effect on the normal secretory patterns of the fluid, we developed a device which allows daily collection of the fluid from unrestricted monkeys. The device was installed intra-abdominally and samples were taken for a period of over 3 months, after which, however, the device was subject to rejection by the animal. The technique and preliminary data obtained are described in this report.

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Slices of testes from normal rats and rats fed diets deficient in essential fatty acids (EFA) were incubated with [1-14C]acetate, [7α-3H]cholesterol and [4-14C]progesterone as substrates. Incorporation of activity in testosterone and androstenedione was measured.

With [1-14C]acetate and [7α-3H]cholesterol as substrates there was an approximate doubling in the yield of testosterone and androstenedione in the testes of EFA-deficient rats, but with [4-14C]progesterone as substrate the differences between groups were not marked. It is postulated that increased biosynthesis of androgens (from acetate and cholesterol substrates) in EFA-deficient animals is perhaps due to structural changes in the mitochondrial wall causing increased permeability of the substrate to the mitochondrial enzymes. No change in the yield of androgens with [4- 14C]progesterone as precursor is perhaps due to non-involvement of mitochondria for synthesis of progesterone→androgens in the rat testis.