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  • Author: G. W. DUNCAN x
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G. W. DUNCAN, L. J. WYNGARDEN and J. C. CORNETTE

Summary.

The effect of nafoxidine HCl and oestradiol on progesterone and oestradiol uptake by rat deciduomata was studied. Rats were ovariectomized and their uteri traumatized; they were subsequently injected for 4 days with 1·5 mg of progesterone and either oestradiol (0·005, 0·05, 0·1, 0·5 or 1·0 μg), or oestradiol (0·05 μg) and nafoxidine HCl (0·5, 5, 50, 500, 1000μg). Tritiated oestradiol or progesterone was injected 4 hr before killing the rats on the last day of treatment.

Uterine horns (traumatized and contralateral non-traumatized cornua) were removed and immediately prepared for the determination of radio-activity. Total non-volatile radio-activity and the radio-activity associated with the protein, ether-soluble and water-soluble fractions were determined. Ether soluble fractions were chromatographed on silica gel; areas associated with major steroid components were isolated and counted for radio-activity.

Both nafoxidine HCl and oestradiol effectively suppress the deciduomata response. The effects of each compound on the above parameters are not comparable in all cases. These differences, and their significance, are discussed.

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Bruce F Kimler, Shawn M Briley, Brian W Johnson, Austin G Armstrong, Susmita Jasti and Francesca E Duncan

Radiation damage due to total body irradiation (TBI) or targeted abdominal radiation can deplete ovarian follicles and accelerate reproductive aging. We characterized a mouse model of low-dose TBI to investigate how radiation affects the follicular and stromal compartments of the ovary. A single TBI dose of either 0.1 Gy or 1 Gy (Cesium-137 γ) was delivered to reproductively adult CD1 female mice, and sham-treated mice served as controls. Mice were euthanized either 2 weeks or 5 weeks post exposure, and ovarian tissue was harvested. To assess the ovarian reserve, we classified and counted the number of morphologically normal follicles in ovarian histologic sections for all experimental cohorts using an objective method based on immunohistochemistry for an oocyte-specific protein (MSY2). 0.1 Gy did not affect that total number of ovarian follicles, whereas 1 Gy resulted in a dramatic loss. At two weeks, there was a significant reduction in all preantral follicles, but early antral and antral follicles were still present. By five weeks, there was complete depletion of all follicle classes. We examined stromal quality using histologic stains to visualize ovarian architecture and fibrosis and by immunohistochemistry and quantitative microscopy to assess cell proliferation, cell death and vasculature. There were no differences in the ovarian stroma across cohorts with respect to these markers, indicating that this compartment is more radio-resistant relative to the germ cells. These findings have implications for reproductive health and the field of fertility preservation because the radiation doses we examined mimic scatter doses experienced in typical therapeutic regimens.