Search Results

You are looking at 1 - 1 of 1 items for

  • Author: Giuseppe Punzi x
  • Refine by access: All content x
Clear All Modify Search
Stefania Muzzachi Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Stefania Muzzachi in
Google Scholar
PubMed
Close
,
Lorenzo Guerra Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Lorenzo Guerra in
Google Scholar
PubMed
Close
,
Nicola Antonio Martino Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy

Search for other papers by Nicola Antonio Martino in
Google Scholar
PubMed
Close
,
Maria Favia Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Maria Favia in
Google Scholar
PubMed
Close
,
Giuseppe Punzi Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Giuseppe Punzi in
Google Scholar
PubMed
Close
,
Fabio Silvestre Section of Veterinary Clinics and Animal Productions, Department of Emergency and Organ Transplantation, University of Bari ‘Aldo Moro’, Valenzano, Bari, Italy

Search for other papers by Fabio Silvestre in
Google Scholar
PubMed
Close
,
Antonio Ciro Guaricci Section of Veterinary Clinics and Animal Productions, Department of Emergency and Organ Transplantation, University of Bari ‘Aldo Moro’, Valenzano, Bari, Italy

Search for other papers by Antonio Ciro Guaricci in
Google Scholar
PubMed
Close
,
Maria Teresa Roscino Section of Veterinary Clinics and Animal Productions, Department of Emergency and Organ Transplantation, University of Bari ‘Aldo Moro’, Valenzano, Bari, Italy

Search for other papers by Maria Teresa Roscino in
Google Scholar
PubMed
Close
,
Ciro Leonardo Pierri Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Ciro Leonardo Pierri in
Google Scholar
PubMed
Close
,
Maria Elena Dell’Aquila Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Maria Elena Dell’Aquila in
Google Scholar
PubMed
Close
,
Valeria Casavola Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Valeria Casavola in
Google Scholar
PubMed
Close
,
Giovanni Michele Lacalandra Section of Veterinary Clinics and Animal Productions, Department of Emergency and Organ Transplantation, University of Bari ‘Aldo Moro’, Valenzano, Bari, Italy

Search for other papers by Giovanni Michele Lacalandra in
Google Scholar
PubMed
Close
, and
Elena Ciani Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari ‘Aldo Moro’, Bari, Italy

Search for other papers by Elena Ciani in
Google Scholar
PubMed
Close

Sperm motility, a feature essential for in vivo fertilization, is influenced by intracellular pH (pHi) homeostasis. Several mechanisms are involved in pHi regulation, among which sodium–hydrogen exchangers (NHEs), a family of integral transmembrane proteins that catalyze the exchange of Na+ for H+ across lipid bilayers. A preliminary characterization of NHE activity and kinetic parameters, followed by analysis of the expression and localization of the protein in ram spermatozoa was performed. NHE activity showed an apparent K m for external Na+ of 17.61 mM. Immunoblotting revealed a molecular mass of 85 kDa. Immunolocalization pattern showed some species-specific aspects, such as positive labeling at the equatorial region of the sperm head. Cariporide, a selective NHE1 inhibitor, significantly reduced pHi recovery (85%). Similarly, exposure to cariporide significantly inhibited different motility parameters, including those related to sperm capacitation. In vitro fertilization (IVF) was not affected by cariporide, possibly due to the non-dramatic, although significant, drop in motility and velocity parameters or due to prolonged exposure during IVF, which may have caused progressive loss of its inhibitory effect. In conclusion, this is the first study documenting, in a large animal model (sheep) of well-known translational relevance, a direct functional role of NHE on sperm pHi and motility. The postulated specificity of cariporide toward isoform 1 of the Na+/H+ exchanger seems to suggest that NHE1 may contribute to the observed effects on sperm cell functionality.

Free access