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P. A. SCHLEIN, M. X. ZARROW, H. A. COHEN, V. H. DENENBERG, and N. P. JOHNSON

Following removal of their olfactory bulbs, virgin female rats consistently attack and kill rat young whereas non-lactating anosmic primiparous animals do not. The present studies were undertaken to compare the effects of bulbectomy on maternal behaviour in the parous and virgin female rat, and to analyse those experiences responsible for the marked behavioural differences towards young seen in these two groups.

Adult female Wistar rats, maintained by random breeding within a closed colony in the laboratory of M. X. Zarrow since 1949 (W/Z strain), were individually housed in 15 × 10 × 7 in. stainless steel cages under a 13-hr light/11-hr dark regimen (lights on at 07.00 hours), and at an ambient temperature of 70 to 74° F. Food and water were freely supplied. The virgin and non-lactating

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A Talmor-Cohen, R Tomashov-Matar, W B Tsai, W H Kinsey, and R Shalgi

Prior to fertilization, the spindle of vertebrate eggs must remain stable and well organized during the second meiotic meta-phase arrest (MII). In a previous study we have determined that the completion of meiosis is a Src family kinase (SFK)-dependent event. In the current study we have used the SFK inhibitors, SU6656 and PP2, and demonstrated that inhibition of SFKs caused the formation of a disorganized spindle. The observation that proper organization of an MII spindle is an SFK-dependent process, combined with our previous finding that Fyn kinase is localized at the microtubules (MTs), prompted us to examine the potential role of Fyn in MT signaling. Our results show an association between Fyn and tubulin, the ability of Fyn to phosphorylate tubulin in vitro and stimulation of meiosis completion by injection of a constitutively active form of Fyn (CAF).

We suggested that SFKs mediate significant functions during the organization of the MII spindle. In view of CAF injection experiments, and of the pronounced concentration of Fyn kinase at the spindle, we propose that Fyn may play an important role in some aspects of the spindle functions, possibly those involving the MTs.