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D. A. PISTENMA, H. C. MEL and N. SNAPIR

Summary.

The interrelationships between the intrinsic motility and electrophoretic properties of fowl spermatozoa were studied in glycinecitrate buffer at temperatures of 3 to 5·5° C using Staflo electrophoresis. Migration patterns obtained with sperm concentrations of 25 × 106 and 250 × 106/ml were similar and gave average electrophoretic mobilities of 1·65±0·10× 10−4 cm/sec/V/cm, corrected to conditions of 1% sucrose at 3° C. At 4° C, there was no significant difference between collection fractions with regard to viable (eosin-negative) spermatozoa. At 5·5° C, however, the lower collection fraction was enriched relative to the upper fraction with regard to motility, viability, fertility and hatchability. For these properties, the lower fraction did not differ from the high quality control. With lower quality starting samples, absolute enrichment may be expected.

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D. A. PISTENMA, N. SNAPIR and H. C. MEL

Summary.

The preservation of the motility and fertilizing capacity of fowl spermatozoa was evaluated under low temperature-low concentration conditions in low-ionic-strength glycine-citrate and glycine-phosphate buffers. Solutions of both buffers at 310 mosmol gave less than a 15% decrement from initial motility values after storage for 6 hr at 3° C in concentrations from 8 to 80× 106 spermatozoa/ml. Fertility results with spermatozoa diluted 1:30 in glycine-citrate buffer, stored for 1½ hr at 3° C and re-concentrated by centrifugation, did not differ from control values, using 1:2 dilution in modified Krebs' solution. These experimental media are thus proved capable of maintaining the vital functions of fowl spermatozoa during storage and for certain desired biophysical studies. They may, therefore, be of potential practical value for work in poultry husbandry and genetics.