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J. A. M. Mattheij
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J. J. M. Swarts
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H. M. H. Hurks
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K. Mulder
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After ovulation, the fertile life of oocytes is short. In the present study, the fertile life of oocytes was studied in relation to the resumption of meiosis. Early on the day of pro-oestrus, meiotic resumption was advanced in rats by a brief infusion of LH; ovulation was induced 8 h later by Ovalyse, a GnRH analogue; rats were mated 13 h after receiving Ovalyse, that is at about the time of ovulation. Rats in which meiosis was not advanced were injected with Ovalyse and mated at various intervals after ovulation. Rats were killed 13 h after mating or on day 20 of pregnancy. In rats in which meiosis was not advanced that were mated around ovulation, fetal survival was about 90%. In rats with meiosis advanced by 8 h and mated around ovulation, only 44% of the ovulated oocytes with advanced meiosis developed into healthy fetuses; mortality before and after implantation was 37 and 19%, respectively, Rats in which meiosis was not advanced that were mated 8–9 h after ovulation had similar fetal survival and similar mortality before and after implantation. Thus ageing of the oocyte may occur either before, or after, ovulation. Preovulatory ageing is related to the resumption of meiosis.

Article Type:
Research Article
DOI:
https://doi.org/10.1530/jrf.0.1000065
Volume/Issue:
Volume 100: Issue 1
Free access
S Tanghe Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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G Vanroose Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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A Van Soom Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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L Duchateau Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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M T Ysebaert Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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P Kerkhofs Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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E Thiry Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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S van Drunen Littel-van den Hurk Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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P Van Oostveldt Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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H Nauwynck Departments of Reproduction, Obstetrics and Herd Health and Physiology, Biochemistry and Biometry, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, 9820 Merelbeke, Belgium, Department of Virology, Veterinary and Agrochemical Research Center, 1180 Brussels, Belgium, Veterinary Virology, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada, Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, 9000 Ghent, Belgium, Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, University of Ghent, 9820 Merelbeke, Belgium

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The purpose of the present study was to identify a potential interference of bovine herpesvirus-1 (BoHV-1) with sperm–oocyte interactions during bovine in vitro fertilization. An inhibition of almost 70% of sperm–zona binding was observed when bovine cumulus-denuded oocytes were inseminated in the presence of 107 50% tissue culture infective dose/ml BoHV-1. The inhibitory effect of BoHV-1 on sperm–zona binding was mediated by an interaction of the virus with spermatozoa, but not with oocytes. Treatment of spermatozoa with BoHV-1, however, did not affect sperm motility and acrosomal status. Antiserum against BoHV-1 prevented the virus-induced inhibition of sperm–zona binding, indicating that BoHV-1 itself affects the fertilization process. In order to investigate which BoHV-1 glycoprotein(s) are responsible for the virus–sperm interaction, BoHV-1 was treated with monoclonal antibodies against the viral glycoproteins gB, gC, gD and gH prior to insemination. Anti-gC completely prevented the inhibitory effect of BoHV-1 on sperm–zona binding, while anti-gD caused a reduction of this inhibition. Further evidence for the involvement of gC and gD in the virus–sperm interaction was provided by the fact that purified gC and gD decreased sperm–zona binding in a dose-dependent way with gC being more effective than gD. These results indicated that BoHV-1 inhibits bovine sperm–zona binding by interacting with spermatozoa. The binding of BoHV-1 to a spermatozoon is mediated by the viral glycoproteins gC and gD, and therefore seems to be comparable with the mechanisms of BoHV-1 attachment to its natural host cell.

Article Type:
Research Article
DOI:
https://doi.org/10.1530/rep.1.00636
Volume/Issue:
Volume 130: Issue 2
Free access