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  • Author: J. D. DUNN x
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R. D. PEPPLER, M. H. BENNETT and J. D. DUNN

Summary.

Thirty-two prepubertal, female rats (Southern Farms) were allocated to intact control, bilateral optic enucleation (blinded), bilateral olfactory bulb removal (anosmic) and blinded—anosmic groups. Olfactory bulbs were surgically removed between 27 and 29 days of age and eyes were removed at 30 days of age. One ovary was removed from each animal between 107 and 112 days of age on Day 2 (metoestrus) of the oestrous cycle. The number of eggs ovulated was determined by flushing the oviducts with normal saline solution. All rats completed one oestrous cycle and were killed at metoestrus of the following cycle. There was no difference in the number of ova shed between the four groups at the time of removal of the first ovary. One cycle later, compensatory ovulation was found at autopsy to have occurred in all animals. Controls ovulated 10·3±0·5 eggs; blinded, 9·6±0·7; anosmic, 10·3±0·3; and blinded—anosmic, 9·7±0·8. Follicular development was quantitatively analysed in both intact and hemispayed blinded and/or anosmic rats. These data suggest that pituitary—ovarian function as evaluated by the number of eggs ovulated is not affected by blinding and/or anosmia.

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R. G. Sutcliffe, D. J. H. Brock, L. V. B. Nicholson and E. Dunn

Summary. Removal of the major maternal serum proteins from second trimester amniotic fluid by antibody affinity chromatography revealed various soluble tissue antigens, of which two were fetal-specific skin proteins and another, of α2-mobility, was specific to the uterus, and was therefore designated alpha-uterine protein (AUP). These proteins could not be detected in maternal serum by antibody–antigen crossed electrophoresis. The concentration of AUP in amniotic fluid reached a maximum between 10 and 20 weeks of gestation, suggesting that there is an influx of uterine protein into the amniotic fluid at this stage of pregnancy.

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G. B. Mann, K. J. Fowler, D. Grail and A. R. Dunn

In this study a rapid, simple and inexpensive procedure is described which allows potential germ-line male mice to be identified with confidence. Spermatozoa recovered by uterine washing following mating with normal female mice was analysed in two ways. First, the patterns of expression of the different isoforms of glucose phosphate isomerase were determined. Since the glucose phosphate isomerase isoforms expressed in embryo stem (ES) cell lines are frequently different from those associated with the host blastocyst, it is possible to determine the proportion of spermatozoa produced by an individual animal that was of ES cell or host–blastocyst origin. Second, DNA of spermatozoa was subjected to polymerase chain reaction (PCR) analysis using primers with specificity for the targeted mutation in the ES cells. The PCR analysis was particularly valuable in identifying germ cell chimaeras in which the contribution of ES-derived spermatozoa was significantly less than that specified by the host blastocyst.