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JAMES J. KENNELLY

Summary.

Sexually mature, male coyotes were injected with [3H]thymidine to estimate (1) the duration of one cycle of the seminiferous epithelium, and (2) epididymal sperm transport time.

The mean duration of spermatogenic stages 1 to 8 was estimated to be 3·4, 2·1, 0·5, 1·4, 1·8, 1·7, 1·5 and 1·3 days, respectively. The total duration of one cycle of the seminiferous epithelium was 13·6 days. Radioactive spermatozoa were observed in the caput epididymidis 40 days after [3H]thymidine injection, but data suggested that they may arrive as early as 37·4 days. Radioactive spermatozoa first appeared in the ejaculate of one male 52 days after [3H]thymidine injection, indicating that the epididymal sperm transport time is about 14 days.

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A. F. McFEE and J. J. KENNELLY

Summary.

A simple and convenient open-surgery procedure for testicular biopsy was tested on rabbits at 1, 3, 5 and 11 months of age. The technique yielded a representative sample of testicular material while damaging less than 1% of the remaining testicular tissue. At least two biopsies can be taken from the sufficiently separated areas of a testis at either 2- or 14-day intervals without the second sample being affected by the first.

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J. J. KENNELLY and R. H. FOOTE

Summary.

The superovulatory response of Dutch-belted does injected with several standard gonadotrophin preparations twice daily for 3 days at 12, 16 and 20 weeks of age and in adult animals was examined to determine the relationship between age, dose and response. The effects of age and the age × treatment interaction were highly significant (P<0·01). At 12 weeks of age 0·31 mg and 0·50 mg fsh/day resulted in overstimulation without ovulation. Prepriming with progesterone apparently counteracted high fsh levels at this age because 0·125 mg fsh with and without progesterone resulted in 16·8 and 1·8 ovulation points respectively. Similar results were obtained at 16 weeks of age, but at 20 weeks of age the number of ovulation points was lower in the progesterone-treated groups. Adult rabbits responded with a mean of 53·7 ovulations following administration of 0·50 mg of fsh. Overstimulation reduced the rate of egg recovery. This was attributed partially to the effect of endogenous oestrogen production on the rate of egg transport and partially to eggs being trapped in follicles.