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  • Author: J. P. O'GRADY x
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J. E. O'GRADY and P. J. HEALD

Summary.

A study has been made of the distribution and spacing of implantation sites in the uterus of the rat at the 5th day of pregnancy. It has been shown that, whereas the implantation sites along the horns are distributed in an entirely random manner, the distances between the sites are significantly non-random. These results are similar to those previously described for the rabbit and the mouse, and rule out the possibility of predicting a site of incipient implantation in the uterus of the rat.

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P. J. HEALD, J. E. O'GRADY and A. J. HOWIE

Summary.

Changes in levels of RNA and DNA in rat uterus have been measured throughout the oestrous cycle in two strains of rats by a method designed specifically for application to uterine tissue. It has been shown that there are significant cyclic variations of RNA and DNA, dry matter and water content, but none in total nitrogen in the uteri of Sprague-Dawley rats with a 5-day cycle, and no significant changes, except in RNA, in the uteri of a Wistar strain with a 4-day cycle. In the Sprague-Dawley rats, RNA/DNA ratios were highest in pro-oestrus and oestrus, and lowest in di-oestrus, the increase in RNA/cell preceding the period of maximum growth and total nitrogen content in the uterus during the cycle.

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P. J. HEALD, J. E. O'GRADY, ANNE O'HARE and MAUREEN VASS

Summary.

The uptake of [5-3H]uridine into RNA and DNA of the cells of the uterine luminal epithelium, stroma and myometrium of the rat has been studied in early pregnancy using a technique for separation of the cell fractions before quantitative analysis. Comparisons of the metabolism between the pregnant and pseudopregnant horn of the unilaterally ovariectomized rat has shown that RNA and DNA synthesis are markedly increased by 04.00 hours on the morning of Day 5 in the pregnant horn. This increased metabolism occurs in all cell fractions and before the zona pellucida is shed. The results are discussed in relation to the onset of the decidual response.

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P. J. HEALD, A. D. T. GOVAN and J. E. O'GRADY

Summary.

An apparatus and procedure is described permitting the ready separation of luminal epithelial and stromal cells (together with the glands) from the rat uterus.

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J. P. O'GRADY, E. I. KOHORN, R. H. GLASS, B. V. CALDWELL, W. A. BROCK and L. SPEROFF

Prostaglandins have been shown to be potent stimulants of cl steroid production in vitro (Pharriss & Wyngarden, 1969; Marsh, 1971; Speroff & Ramwell, 1970), yet prostaglandin F (PGF) given in vivo effectively inhibits progesterone secretion in rats, rabbits, guinea-pigs, sheep and the rhesus monkey (see Pharriss, Tillson & Erickson, 1972, for review). A better understanding of the possible rôle of prostaglandins has been confused by these paradoxical findings. In such studies, it would be of value to have as an experimental model, an in-vitro system in which it is possible to duplicate the inhibitory effects on luteal steroidogenesis seen with the use of PGF administered in vivo. This communication describes an in-vitro method in which sections of rabbit cl are maintained