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JOSEF MATOUSEK

Summary.

Spermagglutinins against seminal and epididymal spermatozoa and against seminal plasma were completely or almost completely absorbed, not only by seminal spermatozoa but also by spermatozoa from the ampullae and from the tail of the epididymis. The agglutinins of the antisera produced by the immunization of epididymal spermatozoa were absorbed very slightly by the fluids of the accessory genital organs. The antibodies against seminal plasma were very well absorbed by the fluids of the epididymis.

On agar plates, anti-seminal plasma antibodies formed five arcs with the seminal plasma of bulls, three arcs with fluid from the seminal vesicles, four arcs with fluid from the ampullae, two arcs with fluid from the tail of the epididymis and one arc with fluid from the head of the epididymis. Seminal spermatozoa, ampullary spermatozoa and seminal plasma, as well as the fluids from these organs, always removed all precipitating antibodies from the antisera. Epididymal spermatozoa absorbed the precipitating antibodies only partially, whereas their fluids did so completely or almost completely. The index of fructolysis of bull seminal spermatozoa was reduced in the medium of the antisera with the antibodies against seminal and epididymal spermatozoa. The decrease varied according to the antiserum used. Any of the substances from the genital tract which were used for the absorption of the antisera, returned the fructolysis processes to normal values.

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JOSEF MATOUSEK

Summary.

We were unable to demonstrate the presence of the erythrocytic antigens of cattle either in the seminal or the epididymal spermatozoa of bulls of our Red Spotted breed. The negative results were not altered by mechanical fragmentation or by enzymic disruption of the spermatozoa.

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JOSEF MATOUSEK

Summary.

In the normal sera of bulls, rams and sows, antibodies agglutinating not only homologous but also heterologous spermatozoa were determined. In the normal sera of some rabbits, only feeble antibodies against boar spermatozoa were present. The sera of bulls and rams also contained spermagglutinins against their own spermatozoa. The absorption of normal sera with homologous spermatozoa suppressed the formation of reactions against homologous and heterologous germ cells. The heterologous spermatozoa only absorbed agglutinins against the spermatozoa of their own species.

Antibodies obtained by immunizing rabbits with bull and ram spermatozoa not only agglutinated homologous spermatozoa, but also produced cross-reactions with germ cells of other species. Immune antibodies against boar spermatozoa agglutinated only homologous spermatozoa. Immune antibodies produced by immunizing rabbits with seminal plasma from a bull agglutinated not only bull but also ram spermatozoa. Antibodies against the seminal plasma of a boar agglutinated only boar spermatozoa. The results of the absorption of immune sera with homologous and heterologous spermatozoa were similar to those obtained with normal sera. Absorption with seminal plasma diminished the titres of antibodies, especially against heterologous spermatozoa.