Search Results
You are looking at 1 - 1 of 1 items for
- Author: Jia-Shun Wu x
- Refine by access: All content x
Search for other papers by Min Zhang in
Google Scholar
PubMed
Search for other papers by Jia-Shun Wu in
Google Scholar
PubMed
Search for other papers by Xiao Han in
Google Scholar
PubMed
Search for other papers by Rui-Jie Ma in
Google Scholar
PubMed
Search for other papers by Jia-Li Xu in
Google Scholar
PubMed
Search for other papers by Ming-Tao Xu in
Google Scholar
PubMed
Search for other papers by Hong-Jie Yuan in
Google Scholar
PubMed
Search for other papers by Ming-Jiu Luo in
Google Scholar
PubMed
Search for other papers by Jing-He Tan in
Google Scholar
PubMed
In brief
Genes expressed in cumulus cells might be used as markers for competent oocytes/embryos. This study identified and validated a new group of cumulus expansion and/or apoptosis-regulating genes, which may be used for selection of quality oocytes/embryos.
Abstract
Studies on the mechanisms behind cumulus expansion and cumulus cell (CC) apoptosis are essential for understanding the mechanisms for oocyte maturation. Genes expressed in CCs might be used as markers for competent oocytes and/or embryos. In this study, both in vitro (IVT) and in vivo (IVO) mouse oocyte models with significant difference in cumulus expansion and CC apoptosis were used to identify and validate new genes regulating cumulus expansion and CC apoptosis of mouse oocytes. We first performed mRNA sequencing and bioinformatic analysis using the IVT oocyte model to identify candidate genes. We then analyzed functions of the candidate genes by RNAi or gene overexpression to select the candidate cumulus expansion and CC apoptosis-regulating genes. Finally, we validated the cumulus expansion and CC apoptosis-regulating genes using the IVO oocyte model. The results showed that while Spp1, Sdc1, Ldlr, Ezr and Mmp2 promoted, Bmp2, Angpt2, Edn1, Itgb8, Cxcl10 and Agt inhibited cumulus expansion. Furthermore, Spp1, Sdc1 and Ldlr inhibited CC apoptosis. In conclusion, by using both IVT and IVO oocyte models, we have identified and validated a new group of cumulus expansion and/or apoptosis-regulating genes, which may be used for selection of quality oocytes/embryos and for elucidating the molecular mechanisms behind oocyte maturation.
