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  • Author: K. R. STEVENS x
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M. HIROI, K. STEVENS and R. GORSKI

Summary.

In normal cyclic female rats, an injection of 16 mg CuSO4/kg induces pseudopregnancy (as determined by uterine traumatization) when given on the day of oestrus, or on the following day. Pentobarbital anaesthesia did not inhibit this effect of CuSO4. Pseudopregnancy, as determined by inhibition of the induction of vaginal cornification by exogenous oestrogen, was also induced by CuSO4 in anovulatory females, provided the injection was given from 6 hr before, to 24 hr after injection of human chorionic gonadotrophin. The latter was necessary since CuSO4 did not induce ovulation in forty experimentally produced anovulatory rats. Cupric sulphate injected on the day of vaginal pro-oestrus in normal females did not alter the incidence of spontaneous ovulation nor the number of ova shed. These data suggest an effect of copper ion in the rat on luteal regulatory mechanisms rather than on ovulatory mechanisms; the site of action cannot, however, be specified.

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K. R. STEVENS, H. D. HAFS and K. T. KIRTON

Summary.

The pH, protein concentration, and amount of oestrous uterine fluid were determined in mature Dutch type rabbits from 1 to 20 weeks after ligation of the uteri. Uterine fluid accumulated at the rate of 2·10 to 4·48 ml per cornu per week. These fluids were serous, slightly turbid, and colourless or slightly yellow. The protein concentration decreased from 5·13 mg/ml after the first week to 1·82 mg/ml after the 20th week of accumulation. The high initial protein concentration may have been due to contamination of the uterine fluid with blood at the time the uterus was ligated. The in-utero pH of fluids was 7·64. A rapid increase in pH with time and handling after removal from the uterus indicated the importance of carbon dioxide in buffering uterine fluids.

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K. R. STEVENS, H. D. HAFS and A. G. HUNTER

Summary.

Fluids were obtained from 185 rabbit uteri 1 to 20 weeks after uterine ligation. The uterine fluid proteins were concentrated with an ultrafilter and characterized by diffusion in agar gel, moving boundary electrophoresis, and immunoelectrophoresis. Eight electrophoretic components were identified by means of moving boundary electrophoresis. One which migrated as a pre-albumin and another which migrated as an alpha-globulin were not found in blood sera. Agar-gel diffusion tests revealed thirteen antigenic components in uterine fluid. Three precipitin lines appeared to be specific to uterine fluid after absorption of guinea-pig antisera to rabbit uterine fluid with rabbit blood sera. However, at least five antigens which could not be found in blood sera were identified in uterine fluid by means of immunoelectrophoresis. The mobilities of two were similar to prealbumins and the mobilities of the remaining three were similar to beta-globulins. The results indicated that at least two classes of proteins, which do not exist in rabbit blood serum, may be found in uterine fluids obtained by ligation.