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K. R. STEVENS, H. D. HAFS and K. T. KIRTON

Summary.

The pH, protein concentration, and amount of oestrous uterine fluid were determined in mature Dutch type rabbits from 1 to 20 weeks after ligation of the uteri. Uterine fluid accumulated at the rate of 2·10 to 4·48 ml per cornu per week. These fluids were serous, slightly turbid, and colourless or slightly yellow. The protein concentration decreased from 5·13 mg/ml after the first week to 1·82 mg/ml after the 20th week of accumulation. The high initial protein concentration may have been due to contamination of the uterine fluid with blood at the time the uterus was ligated. The in-utero pH of fluids was 7·64. A rapid increase in pH with time and handling after removal from the uterus indicated the importance of carbon dioxide in buffering uterine fluids.

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K. T. KIRTON, H. D. HAFS and A. G. HUNTER

Summary.

Four consecutive ejaculates were collected within 1 hr, at weekly intervals for 4 weeks, from each of five mature Holstein bulls to determine the influence of repetitive ejaculation on the levels of some seminal constituents. The pH of the fresh semen and the concentrations of fructose and citric acid in the seminal plasma increased (P<·01) while the concentration and total amount of free amino nitrogen declined from first to fourth ejaculates (P<0·01). Repetitive ejaculation did not significantly influence the concentrations of protein and zinc in the seminal plasma (P ≃ 0·20) or the total citric acid and fructose per ejaculate (P ≃ 0·20). Moving boundary electrophoresis of the proteins and agar-gel-diffusion studies of the antigens in seminal plasma revealed that each protein component observed in first ejaculates was also found in fourth ejaculates. However, the proportion of the protein components in fourth ejaculates differed from that in first ejaculates. The results suggested that the total contribution of the seminal vesicles to the seminal plasma was relatively constant, but their relative contribution increased, from the first to the fourth ejaculate, at least partly at the expense of the contribution of the epididymides.

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CLAUDE DESJARDINS, K. T. KIRTON and H. D. HAFS

Summary.

Bovine follicular fluid was compared with blood serum or blood plasma with respect to the concentration of protein and free amino nitrogen, the number of antigens and the number and mobilities of electrophoretic components. The protein concentration of follicular fluid (7·08 g/100 ml) was less than that of blood serum (9·10 g/100 ml), but no significant difference existed between the values of free amino nitrogen in the two fluids (4·31 and 3·97 mg/100 ml, respectively).

Rabbit antisera to follicular fluid, blood serum or blood plasma gave several precipitin lines (at least 7, 7 and 8 respectively) when reacted with their homologous antigens in agar–gel-diffusion studies. After absorption with blood serum, there was one antigen (presumably fibrinogen) in blood plasma and follicular fluid that was not found in blood serum. These results were substantiated by immuno-electrophoresis.

Moving boundary electrophoresis in five different buffers revealed at least eight components in follicular fluid and blood serum and at least nine in blood plasma. Minor differences were observed in the electrophoretic mobilities of some components found in both follicular fluid and blood serum.

The results showed that the major macromolecular components of follicular fluid and of blood were similar, but that some minor macromolecular ones may differ.

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C. DESJARDINS, K. T. KIRTON and H. D. HAFS

Summary.

Each of twelve rabbits was ejaculated for 5-week periods at frequencies of: once a week (1 × F) ; four times on Friday (4 × F) ; twice on Monday, Wednesday and Friday (2 × MWF) ; and once daily except Sunday (1×M-S). Average sperm outputs/ejaculate (×106) for the four ejaculation frequencies were: 273 for 1 × F; 114, 207, 141 and 80 for first, second, third and fourth ejaculates, respectively, at 4×F; 78 and 128 for first and second ejaculates, respectively, at 2 × MWF; and 86 for 1 × M-S. Repetitive ejaculation significantly increased weekly sperm output (P<0·01). Component of variance analysis of weekly sperm output indicated that differences among bucks were magnified by the more intensive ejaculation frequencies, but not as much as average sperm output.

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K. T. KIRTON, R. J. ERICSSON, J. A. RAY and A. D. FORBES

Summary.

Six, mature, male rhesus monkeys were given U-5897 (3-chloro-1,2-propanediol), 30·0 mg/kg/day, orally. Antifertility efficacy was assessed by comparing pre-treatment fertility (fourteen of twenty-eight matings fertile) with treatment fertility (one of twenty-four matings fertile). The dose was near the anticipated maximum tolerated level, therefore side effects were not unexpected. Three of the six experimental animals showed no detectable toxicity, death of two of the other three was apparently due to bone marrow depression. Sperm motility and morphology were not detectably altered in five of the six animals. Unaltered spermatogenesis and the short time required to attain efficacy in the present experiment imply a post-testicular site of action. Reversibility was demonstrated in three males by post-treatment matings (three of three matings fertile).