Summary. Pronase-resistant low molecular weight stimulators for the activation of proacrosin to acrosin were found in rhesus monkey oviduct fluid collected before, during and after ovulation, but the presence of high concentrations of acrosin inhibitors before and after ovulation partly masked the stimulation in unfractionated fluid. This low molecular weight fraction of oviduct fluid had no detectable esterase or amidase activity by itself, and the stimulating factors were sensitive to digestion by hyaluronidase and chondroitin ABC lyase and were presumed to be glycosaminoglycans. Heparin and hyaluronic acid had similar effects. The presence of soluble glycosaminoglycans at the site of fertilization suggests that they may have a role in capacitation and fertilization.
R. Stambaugh and L. Mastroianni Jr
A. DAVID and L. MASTROIANNI Jr
Cyclic variations in the quality of cervical mucus were evaluated in the monkey. A characteristic pattern was observed. On the day before ovulation maximal fern production was noted, with a dramatic regression in fern pattern in the subsequent day. A consistent correlation with changes in vaginal cytology was not observed. Daily examination of fern pattern provides a reliable index for ovulation timing in the monkey.
R. STAMBAUGH, C. NORIEGA and L. MASTROIANNI Jr
Experiments are described which identify the dialysable corona cell dispersing factor of rabbit oviduct fluid as the bicarbonate ion. In vitro dispersion of the corona cells with bicarbonate ion in media devoid of oviduct fluid or oviduct fluid extracts was also demonstrated. The dispersion process is initiated at bicarbonate ion concentrations of approximately 46 m-equiv/1, while concentrations of 66 m-equiv/1 effect complete corona cell dispersion within a 2-hr incubation period at 37·5° C with no mechanical agitation. Additional evidence that bicarbonate ion is indeed the in vivo corona cell dispersing factor of oviduct fluid was provided by in vivo inhibition of corona cell dispersion by acetazolamide, a carbonic anhydrase inhibitor. It was also observed that follicular ova, in contrast to mature tubal ova, are not invariably denuded with this sequential hyaluronidase and bicarbonate ion treatment.
A. DAVID, B. G. BRACKETT, C-R. GARCIA and L. MASTROIANNI Jr
Forty-six, mature, New Zealand white does were used for collection of fluid from the Fallopian tubes. Ligation of the tubes into four isolated segments was carried out with minimal disturbance to the blood supply, using an operating microscope. The volume of fluid collected decreased from the fimbriated end to the utero-tubal junction.
Analysis of the chemical constituents of the fluid from the first three segments showed that the concentration of sodium, bicarbonate, inorganic phosphate, proteins and lactic acid increased significantly from the fimbriated end to the utero-tubal junction, while chloride concentration decreased significantly. These results support the possibility that the different segments of the oviduct may play specific roles in early events of reproduction.