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SIDNEY SHULMAN, LOUISE MAMROD, RAYMOND W. LANG, MAURICE J. GONDER and WARD A. SOANES

Summary.

Prostatic acid phosphatase can be detected in individual human prostatic fluids by means of gel diffusion antigen-antibody precipitation with specific staining procedures. The fluids can be diluted to various degrees before the reaction fails. This titre, or limiting dilution, ranged from 1 : 64 to 1 : 16,000. It paralleled the results obtained by the King-Armstrong assay of enzyme activity. Gel diffusion analysis revealed the presence of two antigens with acid phosphatase activity.