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  • Author: LUIGI MASTROIANNI Jr x
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LUIGI MASTROIANNI Jr. and RICHARD JONES

Summary.

A system was devised for measuring the oxygen tension within the lumen of the fallopian tube in the unanaesthetized rabbit. After preliminary readings which revealed a mean intraluminal pO2 of 60 mm Hg, ovulation was induced with i.v. HCG. The oxygen tension remained essentially unchanged at the estimated time of ovulation and in the first 3 days after ovulation.

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LUIGI MASTROIANNI Jr. and JAMSHID EHTESHAMZADEH

Summary.

Exposure to tubal fluid in vitro causes loosening of the corona radiata of recently ovulated ova, pretreated with hyaluronidase. Incubation of ova in 0·05 ml of fluid results in denudation of ova to the zona pellucida if mechanical agitation is provided by shaking with admixed sand. Control ova in Krebs-Ringer phosphate solution, Gey's solution, hyaluronidase or rabbit serum are unaltered under similar conditions. Both oestrous and postovulatory fluid are effective. The responsible factor is dialysable, heat-stable and survives lyophilization.

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THOMAS H. CLEWE and LUIGI MASTROIANNI JR.

Summary.

The rate of oviduct secretion in the unanaesthetized rabbit was measured by a volumetric collecting device. After ligation and transection at the uterotubal junction, a polyethylene cannula was secured at the tubal ostium and lead through the lateral abdominal wall to a calibrated glass-lined system. The latter was fixed to the skin and was provided with a vent to the atmosphere and a length of rubber tubing for removal of fluid by needle aspiration. The method was satisfactory in forty of fifty-five rabbits, being maintained for 5 to 66 days. The average duration was 27.2 days. The secretion rate remained stable at a given level in thirty-six subjects. Individual secretion rates ranged between 0.02 and 0.13 ml/hr. Median and mean rates for the group were 0.06 ml/hr. Four animals exhibited two discrete secretion rates during the period of observation. In three animals in which both oviducts were studied, there was no significant difference between the sides. The system permits qualitative and quantitative study of oviduct secretions under various hormonal conditions in a given animal over many days.